Patents by Inventor Perttu Koskinen
Perttu Koskinen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11845974Abstract: The present invention relates to improved methods for producing biosynthetic products in a cascade of bioreactors. In particular the present invention relates to methods and a cascade of bioreactor systems comprising at least two bioreactors and at least one concentration unit.Type: GrantFiled: May 25, 2016Date of Patent: December 19, 2023Assignee: NESTE CORPORATIONInventors: Perttu Koskinen, Heidi Vainio, Miia Laamanen, Raisa Vermasvuori, Reijo Tanner
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Patent number: 10787687Abstract: The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure also relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis with a delignification step using a cationic compound.Type: GrantFiled: December 11, 2014Date of Patent: September 29, 2020Assignee: NESTE OYJInventors: Heidi Vainio, Olavi Myllymaki, Ville Pihlajaniemi, Mika Sipponen, Simo Laakso, Ilkka Lehtomaki, Ossi Pastinen, Perttu Koskinen
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Patent number: 10604777Abstract: The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis and an alkaline delignification step.Type: GrantFiled: December 11, 2014Date of Patent: March 31, 2020Assignee: NESTE OYJInventors: Heidi Vainio, Ville Pihlajaniemi, Mika Sipponen, Ossi Pastinen, Ilkka Lehtomäki, Simo Laakso, Olavi Myllymäki, Perttu Koskinen
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Publication number: 20190359770Abstract: The present invention relates to the preparation of novel sulphur-containing polyamides from renewable sources, and methods for producing the same. The production method involves the preparation of sulphur-containing functional monomers, which can subsequently undergo polycondensation to from either AB- or AABB-type sulphur-containing polyamides. Furthermore, these novel polyamides display superior water barrier/adsorption, better retention of physical properties at elevated temperature (?Tg) and easier processability and polyolefin-compatibility than conventional polyamides. Potential applications for these novel polyamides include high-end electronic devices, organic light-emitting diode devices, components for charge-coupled devices (CCDs) and image sensors (CISs), films and coatings, food packaging films, furniture, appliances, sports equipment, consumer goods, wire and cable, and automotive components.Type: ApplicationFiled: September 7, 2017Publication date: November 28, 2019Applicant: Neste CorporationInventors: Jukka SEPPÄLÄ, Steven SPOLJARIC, Phan Huy NGUYEN, Tomi NYMAN, Perttu KOSKINEN
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Publication number: 20180171371Abstract: The present invention relates to improved methods for producing biosynthetic products in a cascade of bioreactors. In particular the present invention relates to methods and a cascade of bioreactor systems comprising at least two bioreactors and at least one concentration unit.Type: ApplicationFiled: May 25, 2016Publication date: June 21, 2018Applicant: NESTE CORPORATIONInventors: Perttu KOSKINEN, Heidi VAINIO, Miia LAAMANEN, Raisa VERMASVUORI, Reijo TANNER
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Patent number: 9890403Abstract: The present disclosure relates to methods for producing microbial lipids. The present disclosure also relates to methods for producing microbial lipids using inhibitors obtainable from lignocellulosic materials to suppress the proliferation of unwanted microorganisms in the fermentation broth. The method can therefore reduce the risk of having contaminating microbes establish in the system and the cultivation and thus higher yields of microbial lipids may be obtained.Type: GrantFiled: December 11, 2014Date of Patent: February 13, 2018Assignee: NESTE OYJInventors: Heidi Vainio, Mika Sipponen, Simo Laakso, Ossi Pastinen, Ilkka Lehtomäki, Perttu Koskinen, Miia Laamanen
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Patent number: 9708593Abstract: This invention relates to cells and methods for producing lipids using cellulosic carbon source. More specifically the invention relates to oleaginous bacterial cells, wherein genes encoding at least one cellulolytic activity has been introduced. This invention also relates to methods for lipid production by cultivating an oleaginous bacterial strain or strains capable of expressing one or more cellulolytic activity.Type: GrantFiled: October 16, 2013Date of Patent: July 18, 2017Assignee: Neste OyjInventors: Stephan Hetzler, Stefan Uthoff, Daniel Broker, Alexander Steinbuchel, Perttu Koskinen
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Publication number: 20160319310Abstract: The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure also relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis with a delignification step using a cationic compound.Type: ApplicationFiled: December 11, 2014Publication date: November 3, 2016Applicant: NESTE OYJInventors: Heidi VAINIO, Olavi MYLLYMAKI, Ville PIHLAJANIEMI, Mika SIPPONEN, Simo LAAKSO, Ilkka LEHTOMAKI, Ossi PASTINEN, Perttu KOSKINEN
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Publication number: 20160312259Abstract: The present invention relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. In particular, the present invention relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis and an alkaline delignification step.Type: ApplicationFiled: December 11, 2014Publication date: October 27, 2016Applicant: NESTE OYJInventors: Heidi VAINIO, Ville PIHLAJANIEMI, Mika SIPPONEN, Ossi PASTINEN, Ilkka LEHTOMÄKI, Simo LAAKSO, Olavi MYLLYMÄKI, Perttu KOSKINEN
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Publication number: 20160304914Abstract: The present disclosure relates to methods for producing microbial lipids. The present disclosure also relates to methods for producing microbial lipids using inhibitors obtainable from lignocellulosic materials to supress the proliferation of unwanted microorganisms in the fermentation broth. The method can therefore reduce the risk of having contaminating microbes establish in the system and the cultivation and thus higher yields of microbial lipids may be obtained.Type: ApplicationFiled: December 11, 2014Publication date: October 20, 2016Applicant: NESTE OYJInventors: Heidi VAINIO, Mika SIPPONEN, Simo LAAKSO, Ossi PASTINEN, Ilkka LEHTOMÄKI, Heidi KAHELIN, Perttu KOSKINEN, Miia LAAMANEN
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Patent number: 9434962Abstract: Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and/or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and/or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and/or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and/or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.Type: GrantFiled: December 21, 2011Date of Patent: September 6, 2016Assignee: Neste OyjInventors: Perttu Koskinen, Reijo Tanner
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Publication number: 20150322417Abstract: This invention relates to cells and methods for producing lipids using cellulosic carbon source. More specifically the invention relates to oleaginous bacterial cells, wherein genes encoding at least one cellulolytic activity has been introduced. This invention also relates to methods for lipid production by cultivating an oleaginous bacterial strain or strains capable of expressing one or more cellulolytic activity.Type: ApplicationFiled: October 16, 2013Publication date: November 12, 2015Applicant: Neste Oil OyjInventors: Stephan Hetzler, Stefan Uthoff, Daniel Broker, Alexander Steinbuchel, Perttu Koskinen
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Patent number: 9115317Abstract: The invention relates to a method for the recovery of oil from an aqueous solution containing oil and solids, as well as to the use of products obtained by this method. The method of the invention comprises at least the following steps of: Adjusting the oil- and solids-containing aqueous solution to a pH which is acidic, separating an oil- and solids-containing fraction from the acidic aqueous solution with an separation agent which is hydrophilic or assumes a hydrophilic character in response to water, recovering the oil- and solids-containing fraction separated by means of said separation agent, extracting oil away from the solids with a water-insoluble extraction agent, and recovering the oil-containing water-insoluble extraction agent.Type: GrantFiled: February 17, 2012Date of Patent: August 25, 2015Assignee: Neste Oil OyjInventors: Miia Mujunen, Ossi Pastinen, Ilkka Lehtomaki, Simo Laakso, Perttu Koskinen
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Patent number: 8993281Abstract: The present invention relates to a genetically modified Acinetobacter host for lipid production. The Acinetobacter host has been genetically modified to be deficient of one or more of genes A) a gene encoding fatty acyl-CoA reductase (EC1.2.1.n2), wherein said host is capable of increased production of TAGs and/or of total lipids compared to the parent host; and/or B) a gene encoding lipase (EC:3.1.1.3), a gene encoding pyruvate dehydrogenase (EC:1.2.2.2), and/or gene ACIAD 2177, or functional equivalents of any of said genes, wherein said host is capable of increased production of wax esters and/or total lipids compared to the parent host.Type: GrantFiled: December 15, 2011Date of Patent: March 31, 2015Assignee: Neste Oil OyjInventors: Suvi Myllyntausta, Virpi Kivinen, Antti Larjo, Tommi Aho, Perttu Koskinen, Matti Karp, Ville Santala
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Patent number: 8986960Abstract: Disclosed are microorganisms of the genus Cupriavidus or Ralstonia, which are genetically modified to express phosphomannose isomerase (EC5.3.1.8) and facilitated diffusion protein (EC1.3.1.74) for mannose uptake, and optionally mannofructokinase (EC2.7.1.4). The microorganisms also may be genetically modified to express xylose isomerase (EC 5.3.1.5), xylulokinase (E 2.7.1.17) and xylose proton symporter E or a high affinity ABC-transporter. The genetically modified microorganisms are capable of growing on mannose, xylose, arabinose, glucose, or galactose, or a combination thereof as the carbon source.Type: GrantFiled: July 15, 2011Date of Patent: March 24, 2015Assignee: Neste Oil OyjInventors: Shanna Sichwart, Birgit Haschenhermes, Daniel Bröker, Alexander Steinbüchel, Stephan Hetzler, Perttu Koskinen
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Patent number: 8936655Abstract: The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.Type: GrantFiled: December 21, 2011Date of Patent: January 20, 2015Assignee: Neste Oil OyjInventors: Perttu Koskinen, Reijo Tanner
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Patent number: 8697403Abstract: Disclosed are processes for producing lipids for biofuel or lubricant and Streptomyces bacteria used in the processes. The processes include steps whereby bacterial cells of the germs Streptomyces are cultivated in a medium comprising organic waste(s) or residue(s) or mixtures thereof as carbon and/or nutrient source(s), wherein the organic waste(s) or residue(s) comprise lignocellulosic waste(s) or lignocellulosic residue(s), recovering lipids from the cells of the bacteria or from the cultivation medium, and using the recovered lipids or a fraction thereof as biofuel and/or lubricant, or as a starting material for biofuel and/or lubricant production.Type: GrantFiled: May 24, 2011Date of Patent: April 15, 2014Assignee: Neste Oil OyjInventors: Maria Holmback, Miina Lehesto, Perttu Koskinen, Johan-Fredrik Selin
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Publication number: 20120220509Abstract: The invention relates to a method for the recovery of oil from an aqueous solution containing oil and solids, as well as to the use of products obtained by this method. The method of the invention comprises at least the following steps of: Adjusting the oil- and solids-containing aqueous solution to a pH which is acidic, separating an oil- and solids-containing fraction from the acidic aqueous solution with an separation agent which is hydrophilic or assumes a hydrophilic character in response to water, recovering the oil- and solids-containing fraction separated by means of said separation agent, extracting oil away from the solids with a water-insoluble extraction agent, and recovering the oil-containing water-insoluble extraction agent.Type: ApplicationFiled: February 17, 2012Publication date: August 30, 2012Applicant: NESTE OIL OYJInventors: Miia Mujunen, Ossi Pastinen, Ilkka Lehtomaki, Simo Laakso, Perttu Koskinen
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Publication number: 20120159840Abstract: The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.Type: ApplicationFiled: December 21, 2011Publication date: June 28, 2012Applicant: NESTE OIL OYJInventors: Perttu Koskinen, Reijo Tanner
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Publication number: 20120159839Abstract: Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and/or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and/or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and/or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and/or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.Type: ApplicationFiled: December 21, 2011Publication date: June 28, 2012Applicant: NESTE OIL OYJInventors: Perttu Koskinen, Reijo Tanner