Abstract: The method presented here is used to determine the volume dispensed by any liquid handling device (automated or manual) . A reference curve is first generated by spectroscopically reading a fixed-volume set of known, variable-concentration derivatives of a single dye. During testing the liquid handling device dispenses a yet undetermined volume of known-concentration dye into a known volume of diluent which results in a new dye concentration (resultant concentration). The absorbance of the resultant concentration is then compared to the absorbance vs concentration relationship of the earlier generated reference curve to determine the volume of dye (hence volume) dispensed by the liquid handling device. This method is an alternative to the dual dye and gravimetric volume verification methodologies. It considers and corrects for the uncertainties found in a traditional single dye approach as stated in the IWA-15 ISO standard.

Abstract: A method is provided for use in solid phase chemical synthesis such as in the synthesis of polypeptides, peptoids, and other molecules synthesized by solid phase methods. The method is used to identify compounds having activity against a selected target, wherein the compounds are present in a mixture obtained from a combinatorial library. A bead distributor probe is also provided. The probe is used to extract beads from a population of beads, and then deliver the bead to a selected location. A capillary bead insert is also provided, as well as a bead distribution system which includes both a bead distributor probe and a capillary bead insert.

Abstract: A method is provided for use in solid phase chemical synthesis such as in the synthesis of polypeptides, peptoids, and other molecules synthesized by solid phase methods. The method is used to identify compounds having activity against a selected target, wherein the compounds are present in a mixture obtained from a combinatorial library. A bead distributor probe is also provided. The probe is used to extract beads from a population of beads, and then deliver the bead to a selected location. A capillary bead insert is also provided, as well as a bead distribution system which includes both a bead distributor probe and a capillary bead insert.