Patents by Inventor Peter A. Ivanov
Peter A. Ivanov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8257945Abstract: A method of search for and identification of a eukaryotic IRES element active in cap-independent translation of RNA in eukaryotic cells is provided, comprising the following steps: i) screening eukaryotic mRNA sequences or corresponding DNA sequences for a potential IRES element having a block of nucleotides having: a) a length of at least 30 nucleotides; b) an adenine nucleotide content of at least 40 mol-%; and c) a pyrimidine nucleotide content of less than 40 mol-%; ii) inserting said potential IRES element into a linear dicistronic construct between an upstream gene and a downstream GUS reporter gene, whereby said potential IRES element is positioned for IRES-dependent translation of said downstream GUS gene and whereby said upstream gene is preceded by a stable hairpin structure to prevent IRES-independent translation of said genes; and iii) testing said potential IRES element for IRES-dependent translation of said GUS gene in a rabbit reticulocyte lysate or in a wheat germ extract in vitro translationType: GrantFiled: September 3, 2002Date of Patent: September 4, 2012Assignee: Icon Genetics, Inc.Inventors: Joseph Atabekov, Yurii Dorokhov, Maxim Skulachev, Peter Ivanov, Yuri Gleba
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Patent number: 8192984Abstract: A method of producing a nucleic acid sequence having an adenine-rich nucleic acid block of at least 25 nucleotides in length is provided. The nucleic acid sequence that is produced is capable of initiating translation at an internal ribosome entry site (IRES) and the adenine-rich nucleic acid block comprises from 40 to 100 mol-% adenine.Type: GrantFiled: September 3, 2002Date of Patent: June 5, 2012Assignee: Icon Genetics, Inc.Inventors: Joseph Atabekov, Yurii Dorokhov, Maxim Skulachev, Peter Ivanov, Yuri Gleba
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Patent number: 8003381Abstract: This invention provides a process of amplification and/or expression of a sequence of interest in a cell by providing for generating within the cell at least one amplicon by trans-splicing between an RNA sequence designed for being capable of trans-splicing and a target RNA, whereby the amplicon is capable of amplifying in the cell and capable of expressing a sequence of interest.Type: GrantFiled: April 30, 2002Date of Patent: August 23, 2011Assignee: Icon Genetics GmbHInventors: Victor Klimyuk, Peter Ivanov, Sylvestre Marillonnet, Meinhart Zenk, Yuri Gleba
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Patent number: 7763458Abstract: The invention describes virus-based amplification vectors for plants containing additional plant-specific internal ribosome entry site (IRES) element(s) allowing for a polycistronic translation and a cap-independent translation of: a) heterologous gene(s); b) whole viral genome or c) viral subgenomic RNAs. Said IRES elements are of plant viral origin, or they are isolated from other organisms or engineered using different synthesis procedures. Said IRES element(s) and said heterologous gene(s) are inserted into amplification vectors and allow for the expression of said heterologous gene(s) in the absence of additional viral promoters, in particular, said expression is achieved through cap-independent translation.Type: GrantFiled: October 8, 2001Date of Patent: July 27, 2010Assignee: Icon Genetics GmbHInventors: Yuri Gleba, Yurii Dorokhov, Peter Ivanov, Joseph Atabekov
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Patent number: 7088243Abstract: Disclosed is a method of intruder's detection, comprising of using of more than 1 sensor, represented, for example, by video cameras that are arranged with fixed spatial orientation for stereo detector formation at that 2D distributions of light intensity that form stereo images registered by stereo detector are transmitting to blocks of processing, for example, blocks of digital processing at that mentioned blocks of digital processing perform processing of sequence of stereo images with different frequencies (high and low), to determine the presence of intruder, it's position and speed of transference.Type: GrantFiled: March 30, 2004Date of Patent: August 8, 2006Assignee: S1 CorporationInventors: Mun Wang Jin, Lee Yong Jin, Alexander B. Murynin, Victor D. Kuznetsov, Peter A. Ivanov, Il-Jun Jeong
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Publication number: 20050221323Abstract: This invention provides process of amplification and/or expression of a sequence of interest in a cell by providing for generating within said cell at least one amplicon by trans-splicing between an RNA sequence designed for being capable of trans-splicing and a target RNA, whereby said amplicon is capable of amplifying in said cell and capable of expressing a sequence of interest.Type: ApplicationFiled: April 30, 2002Publication date: October 6, 2005Applicant: Icon Genetics AGInventors: Victor Klimyuk, Peter Ivanov, Sylvestre Marillonnet, Meinhart Zenk, Yuri Gleba
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Patent number: 6933378Abstract: A primary object of this invention is to provide a method which will enable to coexpress simultaneously two (or more) desired genes in plant, animal or yeast cells, in transgenic plants and animals, or in vitro, in plant cell-derived or animal cell-derived translation systems. This objection is to be accomplished by utilizing sequence elements derived from RNAs of a tobamovirus upstream of MP gene or CP gene termed here as IRESMP and IRESCP , respectively. The method of this invention involves the construction of a recombinant nucleic acid sequence which comprises a specific transcriptional promoter, a first gene expressible in eukaryotic cells linked to said transcriptional promoter, IRESMP or IRESCP located 3? to the first gene and a second gene expressible in eukaryotic cells, located 3? to IRES sequence such that the second gene is placed under the transcriptional control of IRES sequence originated from tobamovirus genome.Type: GrantFiled: July 25, 2001Date of Patent: August 23, 2005Inventors: Joseph Atabekov, Timo Korpela, Yurii Dorokhov, Peter Ivanov, Maxim Skulachev, Nina Rodionova, Olga Karpova
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Publication number: 20050059004Abstract: A method of creating a nucleic acid sequence having an adenine-rich nucleic acid block of at least 25 nucleotides in length is provided, whereby said nucleic acid sequence is capable of causing translation by internal ribosome entry (IRES element). Said adenine-rich nucleic acid block comprises from 40 to 100 mol-% adenine.Type: ApplicationFiled: September 3, 2002Publication date: March 17, 2005Inventors: Joseph Atabekov, Yurii Dorokhov, Maxim Skulachev, Peter Ivanov, Yuri Gleba
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Publication number: 20050014150Abstract: A method of search for and identification of a eukaryotic IRES element active in cap-independent translation of RNA in eukaryiotic cells is provided, comprising the following steps: i)screening eukaryotic mRNA sequences or corresponding DNA sequences for a potential IRES element having a block of nucleotides having: a) a length of at least 30 nucleotides; b) an adenine nucleotide content of at least 40 mol-%; and c) a pyrimidine nucleotide content of less than 40 mol-%; ii) inserting said potential IRES element into a linear dicistronic construct between an upstream gene and a downstream GUS reporter gene, whereby said potential IRES element is positioned for IRES-dependent translation of said downstream GUS gene and whereby said upstream gene is preceded by a stable hairpin structure to prevent IRES-independent translation of said genes; and iii) testing said potential IRES element for IRES-dependent translation of said GUS gene in a rabbit reticulocyte lysate or in a wheat germ extract in vitro translationType: ApplicationFiled: September 3, 2002Publication date: January 20, 2005Inventors: Joseph Atabekov, Yurii Dorokhov, Maxim Skulachev, Peter Ivanov, Yuri Gleba
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Publication number: 20040239761Abstract: Disclosed is a method of intruder's detection, comprising of using of more than 1 sensor, represented, for example, by video cameras that are arranged with fixed spatial orientation for stereo detector formation at that 2D distributions of light intensity that form stereo images registered by stereo detector are transmitting to blocks of processing, for example, blocks of digital processing at that mentioned blocks of digital processing perform processing of sequence of stereo images with different frequencies (high and low), to determine the presence of intruder, it's position and speed of transference.Type: ApplicationFiled: March 30, 2004Publication date: December 2, 2004Applicant: S1 CORPORATIONInventors: Mun Wang Jin, Lee Yong Jin, Alexander B. Murynin, Victor D. Kuznetsov, Peter A. Ivanov, Il-Jun Jeong
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Publication number: 20040055037Abstract: The invention describes virus-based amplification vectors for plants containing additional plant-specific internal ribosome entry site (IRES) element(s) allowing for a polycistronic translation and a cap-independent translation of: a) heterologous gene(s); b) whole viral genome or c) viral subgenomic RNAs. Said IRES elements are of plant viral origin, or they are isolated from other organisms or engineered using different synthesis procedures. Said IRES element(s) and said heterologous gene(s) are inserted into ampification vectors and allow for the expression of said heterologous gene(s) in the absence of additional viral promoters, in particular, said expression is achieved through cap-independent translation.Type: ApplicationFiled: October 22, 2003Publication date: March 18, 2004Inventors: Yuri Gleba, Yurii Dorokhov, Peter Ivanov, Joseph Atabekov
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Patent number: 6376745Abstract: The present invention is drawn to a recombinant nucleic acid having (a) a transcriptional promoter; (b) a first plant-expressible structural gene linked to the transcriptional promoter, (c) a cDNA sequence element having an internal ribosome entry site of tobamovirus moving protein (MP) gene (IRESmp) which is located 3′ to the first plant-expressible structural gene, and (d) a second plant-expressible gene located 3′ to the (IRESmp) such that the second gene is placed under the translational control of (IRESmp); wherein the first plant-expressible gene, (IRESmp) and the second plant-expressible gene are transcribed under the action of the transcriptional promoter to give a primary transcript, and the first plant expressible gene of the primary transcript is able to translate by ribosome scanning mechanism and the second plant expressible gene of the primary transcript is capable of translation under the action of (IRESMP).Type: GrantFiled: December 16, 1999Date of Patent: April 23, 2002Inventors: Joseph Atabekov, Timo Korpela, Yurii Dorokhov, Peter Ivanov, Maxim Skulachev, Nina Rodionova, Olga Karpova
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Publication number: 20020034814Abstract: A primary object of this invention is to provide a method which will enable to coexpress simultaneously two (or more) desired genes in plant, animal or yeast cells, in transgenic plants and animals, or in vitro, in plant cell-derived or animal cell-derived translation systems. This objection is to be accomplished by utilizing sequence elements derived from RNAs of a tobamovirus upstream of MP gene or CP gene termed here as IRESmp and IREScp , respectively. The method of this invention involves the construction of a recombinant nucleic acid sequence which comprises a specific transcriptional promoter, a first gene expressible in eukaryotic cells linked to said transcriptional promoter, IRESmp or IREScp located 3′ to the first gene and a second gene expressible in eukaryotic cells, located 3′ to IRES sequence such that the second gene is placed under the transcriptional control of IRES sequence originated from tobamovirus genome.Type: ApplicationFiled: July 25, 2001Publication date: March 21, 2002Inventors: Joseph Atabekov, Timo Korpela, Yurii Dorokhov, Peter Ivanov, Maxim Skulachev, Nina Rodionova, Olga Karpova
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Patent number: 4495789Abstract: A device for use with a hydraulic cylinder or like for straightening frame members, which converts the extension stroke of the cylinder to a pulling action. The device has a pair of interengaging outer members which are formed to define an elongate cage. The interengaging members are slidably connected to one another for relative movement in the direction of the longitudinal axis of the cage. Retaining means are mounted on the interengaging outer members, which are adapted to removably retain a hydraulic cylinder within the cage aligned along the central longitudinal axis, so that extension of the cylinder causes a contraction of the interengaging member along the direction of the longitudinal axis. The interengaging members are adapted at opposite ends for connection to a frame member to be straightened.Type: GrantFiled: October 18, 1982Date of Patent: January 29, 1985Inventor: Peter Ivanov