Abstract: Disclosed are methods and kits for genome-wide methylation of GpC sites and for genome-wide chromatin structural determination. Specifically, the methods and kits of the present invention make possible the simultaneous determination of endogenous DNA methylation state and chromatin architecture across the entire genome.

Abstract: Exemplary embodiments of the invention provide an apparatus and method for generating power from a renewable source of environmental water. The apparatus includes an upstanding structure adapted for support on ground adjacent to the renewable source of water having an elevated region relative to the ground. A conduit for water extends between the elevated region of the water supply and a water inlet. A gravity-operated energy converter supported by the structure is provided that causes water to descend vertically between the inlet and an outlet adjacent the ground while utilizing the weight of the water thus descending to drive at least one movable element and thereby produce power. Additional energy may be provided from buoyancy by causing empty and inverted water containers to ascend through a column of water fed by the renewable source of water.

Abstract: Particular embodiments provide novel and clinically useful DNA methylation predictors of hormone receptor status, and predictors of response to endocrine (e.g., hormonal) and non-endocrine breast cancer therapy. The ESR1 gene, encoding the estrogen receptor (ER) alpha proved to be the preferred predictor of progesterone receptor (PR) status, while methylation of the PGR gene, encoding PR, was the preferred predictor of ER status. ESR1 methylation outperformed hormone receptor status as a predictor of clinical response in patients treated with antiestroges (e.g., tamoxifen), while promoter methylation of the CYP1B1 gene, encoding a tamoxifen and estradiol metabolizing cytochrome P450, predicted response differentially in tamoxifen-treated and non-treated patients. High levels of promoter methylation of the ARH1 gene, encoding a RAS-related small G-protein, were shown to be preferred predictors of better survival in patients who had not received tamoxifen therapy.

Abstract: Zebularine has hypomethylating activity, and can be used to inhibit, reverse, and/or reduce DNA methylation in vivo and in vitro. Methods are provided for treating methylation-linked conditions through the application of 2-pyrimidinone derivatives, such as Zebularine. Compositions, including pharmaceutical compositions, comprising such derivatives are also provided. Also provided are kits for use in inhibiting DNA methylation, which kits include an amount of a 2-pyrimidinone derivative.

Abstract: Methods and systems are disclosed to facilitate holistic management of different types of IT resources within and work associated with an IT infrastructure. In one example, a method includes defining a number of parameters related to IT resources, associating an IT resource with at least one of the parameters, and aggregating a number of IT resources as an IT infrastructure. Each of the IT resources of the IT infrastructure is associated with at least one of the parameters. In another example, a method includes defining a number of parameters related to a number of different types of IT resources, associating two or more of the parameters with one another, and aggregating the parameters in an IT resource definitional structure by which any one of the types of IT resources can be defined by associating the IT resource with at least one of the parameters.

Abstract: The present invention relates in general to microRNA profiling in disease. More specifically, the invention provides for methods and compositions of microRNA to inhibit the growth and formation of tumors.

Abstract: The present invention relates in general to microRNAs (miRNAs). More specifically, the invention relates to expression vectors comprising multiple miRNA families and clusters capable of targeting multiple oncogenic pathways.

Abstract: There is disclosed 103 novel methylation-altered DNA sequences (“marker sequences”) that have distinct methylation patterns in cancer, compared to normal tissue. In many instances, these marker sequences represent novel sequences not found in the GenBank data base, and none of these marker sequences have previously been characterized with respect to their methylation pattern in human cancers including, but not limited to those of bladder and prostate. These 103 sequences have utility as diagnostic, prognostic and therapeutic markers in the treatment of human cancer, and as reagents in kits for detecting methylated CpG-containing nucleic acids.

Abstract: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.

Abstract: Particular embodiments provide novel and clinically useful DNA methylation predictors of hormone receptor status, and predictors of response to endocrine (e.g., hormonal) and non-endocrine breast cancer therapy. The ESR1 gene, encoding the estrogen receptor (ER) alpha proved to be the preferred predictor of progesterone receptor (PR) status, while methylation of the PGR gene, encoding PR, was the preferred predictor of ER status. ESR1 methylation outperformed hormone receptor status as a predictor of clinical response in patients treated with antiestroges (e.g., tamoxifen), while promoter methylation of the CYP1B1 gene, encoding a tamoxifen and estradiol metabolizing cytochrome P450, predicted response differentially in tamoxifen-treated and non-treated patients. High levels of promoter methylation of the ARHI gene, encoding a RAS-related small G-protein, were shown to be preferred predictors of better survival in patients who had not received tamoxifen therapy.

Abstract: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.

Abstract: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.

Abstract: Abstract There is disclosed a method to measure the fraction of DNA molecules that ishemi-methylated at a specific CpG dinucleotide in a particular DNA sequence, in a pool of DNA molecules having mixed DNA methylation states at said CpG dinucleotide. There is also disclosed methods to calculate the rates of de novo-, and maintenance-methylation per cell division that occur at said CpG dinucleotide. Additionally, there is disclosed methylation kits for use in measuring and quantitating hemi-methylation, and for calculating the rates of de novo-, and maintenance-methylation.

Abstract: An identification device for eyeglass frames is provided. The identification device includes an identification sleeve adapted to couple with an eyeglass frame. This identification sleeve includes an identification marking. A non-toxic protective sleeve is superimposed over the identification sleeve and at least a portion of an eyeglass frame. This device does not accumulate dirt and bacteria and easily attaches to an eyeglass frame.

Abstract: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.

Abstract: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.

Abstract: An identification device for eyeglass frames is provided. The identification device includes an identification sleeve adapted to couple with an eyeglass frame. This identification sleeve includes an identification marking. A non-toxic protective sleeve is superimposed over the identification sleeve and at least a portion of an eyeglass frame. This device does not accumulate dirt and bacteria and easily attaches to an eyeglass frame.

Abstract: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.

Abstract: A new scene-based wavefront sensor is useful in a device as, for example, a telescope or camera that provides information of aberrations (e.g., out-of-focus condition) directly from light received from a scene in object space being scanned by movement of either the wavefront sensor or elements in the scene. The wavefront sensor includes an imaging system defining a predetermined pupil, a light opaque material having a surface and defining a probe hole (pinhole) therethrough, a linear image sensor array, a relay lens, and a pupil sensor array including a plurality of light sensors. The probe hole and image sensor array are disposed at a focal plane of the imaging system while the pupil sensor array is disposed at a focal plane of the pupil of the imaging system.

Abstract: A rotary brush mounting comprises support structure mountable from a sweeping machine and carrying support means for angular movement about pivotal axes at right angles to one another in a universal manner and a driven rotary brush depending from the support means and normally rotatable about a substantially vertical axis but capable of sideways and back and forth inclining movement with the support means so as to follow varying formation or irregularities of a road or similar surface in order to maintain effective sweeping contact therewith. The support structure may consist of a bracket having a pivoted arm extending for swinging movement and carrying the support means which latter may consist of a forked member longitudinally pivoted on the arm and carrying between its limbs for transverse pivotal movement a support member for the brush together with a direct motor drive to the latter.