Abstract: This disclosure describes methods of preparing a decellularized Descemet's membrane and an isolated Descemet's membrane, methods of using an isolated Descemet's membrane, and tissues prepared using an isolated Descemet's membrane. This disclosure further describes a composition that includes an isolated Descemet's membrane. In some embodiments, the tissues and methods described herein may be used to treat a limbal stem cell deficiency or as an ocular surface bandage.

Abstract: This disclosure describes a Descemet's membrane endothelial keratoplasty (DMEK) graft prepared using paired incisions; compositions including the incised DMEK graft; an injector including the incised DMEK graft; a viewing chamber including the incised DMEK graft, methods of preparing the incised DMEK graft; and methods of using the DMEK graft, the compositions, the injectors, and the viewing chambers including to facilitate placement of DMEK grafts during cornea transplant surgery.

Abstract: A graft injector assembly includes a coating disposed on an interior surface of an injector body to form a lumen. The lumen is structured to store a graft comprising corneal tissue and to allow the graft to exit distally from the injector body, preferably with minimal or no damage to the corneal tissue. The corneal tissue may include a Descemet membrane and endothelial cells, which may be used in Descemet Membrane Endothelial Keratoplasty (DMEK). In one example, the assembly includes an organosilane coating disposed on a glass injector body.

Abstract: Compositions, devices, and systems comprising a corneal tissue carrier, wherein the corneal tissue carrier comprises a corneal tissue sample and a fluid; wherein the fluid comprises resazurin. Additionally, methods including measuring cell viability.

Abstract: This disclosure describes methods of preparing a decellularized Descemet's membrane and an isolated Descemet's membrane, methods of using an isolated Descemet's membrane, and tissues prepared using an isolated Descemet's membrane. This disclosure further describes a composition that includes an isolated Descemet's membrane. In some embodiments, the tissues and methods described herein may be used to treat a limbal stem cell deficiency or as an ocular surface bandage.

Abstract: This disclosure describes a Descemet's membrane endothelial keratoplasty (DMEK) graft prepared using paired incisions; compositions including the incised DMEK graft; an injector including the incised DMEK graft; a viewing chamber including the incised DMEK graft, methods of preparing the incised DMEK graft; and methods of using the DMEK graft, the compositions, the injectors, and the viewing chambers including to facilitate placement of DMEK grafts during cornea transplant surgery.

Abstract: Blood component processing systems and methods are coupled to a blood component processing device. The systems and methods include a fluid circuit that comprises at least one fluid pressure actuated pump station, at least one fluid flow path communicating with the pump station, and at least one valve in the fluid flow path. A control module is sized and configured to interact with the fluid circuit. The control module includes an actuator to selectively apply fluid pressure to the pump station in concert with operation of the valve to pump fluid through the fluid flow path in response to a control program. A controller coupled to the control module includes the control program and a fluid circuit integrity test function.

Abstract: Blood component processing systems and methods are coupled to a blood component processing device. The systems and methods include a fluid circuit that comprises at least one fluid pressure actuated pump station, at least one fluid flow path communicating with the pump station, and at least one valve in the fluid flow path. A control module is sized and configured to interact with the fluid circuit. The control module includes an actuator to selectively apply fluid pressure to the pump station in concert with operation of the valve to pump fluid through the fluid flow path in response to a control program. A controller coupled to the control module includes the control program and a fluid circuit integrity test function.

Abstract: Blood component processing systems and methods are coupled to a blood component processing device. The systems and methods include a fluid circuit that comprises at least one fluid pressure actuated pump station, at least one fluid flow path communicating with the pump station, and at least one valve in the fluid flow path. A control module is sized and configured to interact with the fluid circuit. The control module includes an actuator to selectively apply fluid pressure to the pump station in concert with operation of the valve to pump fluid through the fluid flow path in response to a control program. A controller coupled to the control module includes the control program and a fluid circuit integrity test function.

Abstract: Blood component processing systems and methods are coupled to a blood component processing device. The systems and methods include a fluid circuit that comprises at least one fluid pressure actuated pump station, at least one fluid flow path communicating with the pump station, and at least one valve in the fluid flow path. A control module is sized and configured to interact with the fluid circuit. The control module includes an actuator to selectively apply fluid pressure to the pump station in concert with operation of the valve to pump fluid through the fluid flow path in response to a control program. A controller coupled to the control module includes the control program and a fluid circuit integrity test function.

Abstract: There is provided a child safety seat and a child safety seat system that are adapted for use in automobiles, watercraft, and/or aircraft. The child safety seat is adapted to float in water such that a child secured in the safety seat is held completely or almost completely above the surface of the water. The child safety seat has a “self-righting” capability such that the seat will turn top-side up in water. The child safety seat has a handle that is integral to the self-righting capability of the child safety seat. In addition, the child safety seat system includes a base that may be used, in particular, in a watercraft and that is adapted to secure the child safety seat such that the child safety seat cannot be shifted horizontally, but can be moved vertically. Alternatively, the base may include a releasable locking assembly, particularly for use in automobiles or aircraft.

Abstract: There is provided a child safety seat and a child safety seat system that are adapted for use in automobiles, watercraft, and/or aircraft. The child safety seat is adapted to float in water such that a child secured in the safety seat is held completely or almost completely above the surface of the water. The child safety seat has a “self-righting” capability such that the seat will turn top-side up in water. The child safety seat has a handle that is integral to the self-righting capability of the child safety seat. In addition, the child safety seat system includes a base that may be used, in particular, in a watercraft and that is adapted to secure the child safety seat such that the child safety seat cannot be shifted horizontally, but can be moved vertically. Alternatively, the base may include a releasable locking assembly, particularly for use in automobiles or aircraft.