Abstract: The invention describes a method and its use for producing complex multienzymatical, storage resistant reaction mixtures for synthesizing, modifying or analyzing polypeptides and optionally nucleic acids characterized in that native or artificial enzymatical, active protein mixtures with reaction buffers, cofactors and substrates are prepared so that they are ready for use and are storage resistant such that only user-specific key components (e.g. mRNS) are missing to start the desired enzymatical reaction(s). In the method a stabilizer is added to the reaction mixtures in the solution which, on the one hand, increases the reacting capacity of the multienzymatical systems and, on the other hand, protects the unstable reaction components from losing their biological activity or their biologically active structure while being made storage resistant and during storage. The reaction mixture is made storage resistant by being easily freeze dried under a vacuum and then durably stored at 4-10° C.

Abstract: The subject of the invention are formulations not containing chaotropic components for isolating nucleic acids with binding to a solid phase, in particular of DNA, from optional complex starting materials and quantities containing a lysis/binding buffer system which comprises at least one antichaotropic salt component, a solid phase and wash and elution buffers known as such. The lysis/binding buffer system may be an aqueous solution or a solid formulation in reaction vessels ready for use. All carriers used for isolation by means of chaotropic reagents, preferably glass fiber mats, glass membranes, silica carriers, ceramics, zeolites or materials showing negatively functionalised surfaces or chemically modified surfaces which may be converted to a negative charge potential may serve as a solid phase.

Abstract: Disclosed is a device for a simultaneous multiple isolation and purification of nucleic acids from biological material based on binding nucleic acid to mineral carrier particles. The device includes a square-shaped plastic case, a shaking apparatus with sample vessels, and a shielding for the motor and shaking apparatus.

Abstract: Method and apparatus for carrying out biochemical reactions in particular for cell-free polypeptide biosynthesis. A bioreactor has, within a housing, a reaction chamber containing a producing system as well as a supply chamber separated from the reaction chamber by means of a semi-permeable membrane and containing a liquid serving as supply medium for the producing system. The supply chamber is subdivided within the bioreactor into a first partial chamber and a second partial chamber which are each connected to the reaction chamber via a semi-permeable supply membrane and which are connected to each other by means of a connection conduit.

Abstract: The invention concerns a method and device for the rapid, simultaneous isolation of genomic desoxyribonucleic acid (DNA) and cellular total ribonucleic acid (RNA), free of genomic DNA from various starting materials. The fields of application are molecular biology, biochemistry, gene technology (in particular gene therapy), medicine, biomedical diagnosis, veterinary medicine, food analysis and all related fields. The method proposed is characterized in that materials containing DNA and RNA are lysed in a special buffer, the lysate incubated with a mineral carrier, the carrier with the DNA bound to it separated off and washed with buffer solution, and the DNA subsequently separated from the carrier with a buffer of lower salt concentration. The lysate left after separating off the DNA bound to the carrier is mixed with phenol, chloroform and sodium acetate in defined proportions, the phases allowed to separate, and the total RNA precipitated from the aqueous phase by adding isopropanol.

Abstract: A universal process is disclosed for extracting and purifying nucleic acids from extremely small amounts of highly contaminated various biological and other starting materials. The invention has applications in forensic medicine, medical diagnosis, molecular biology, biochemistry, genetic technology and all related fields. The process is characterized in that nucleic acid-containing materials are lysed, the lysate is incubated with a non-porous, non-structured, highly disperse, homogeneous and chemically pure SiO.sub.2 substrate, the substrate is isolated with the bound nucleic acids and washed with a buffer solution, then the nucleic acids are dissolved from the substrate by a buffer with a lower salt concentration. Lysis of the material and nucleic acid immobilization are preferably carried out in a reaction vessel. The substrate particles have a size of 7-40 nm, preferably 40 nm, and a specific surface from 50-300 g/m.sup.2, preferably 50 g/m.sup.2.