Abstract: A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed. More particularly, a biologically active polypeptide fused to a protein sequence that mediates the induction of RPBLA formation is expressed and accumulated in host cells after transformation with an appropriate vector. The eukaryotic host cell does not produce protein bodies in the absence of the fusion protein. Methods for preparing and using the RPBLAs and the fusion protein are also disclosed, as are nucleic acid molecules that encode the fusion proteins.

Abstract: A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed. More particularly, a biologically active polypeptide fused to a protein sequence that mediates the induction of RPBLA formation is expressed and accumulated in host cells after transformation with an appropriate vector. The eukaryotic host cell does not produce protein bodies in the absence of the fusion protein. Methods for preparing and using the RPBLAs and the fusion protein are also disclosed, as are nucleic acid molecules that encode the fusion proteins.

Abstract: A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed. More particularly, a biologically active polypeptide fused to a protein sequence that mediates the induction of RPBLA formation is expressed and accumulated in host cells after transformation with an appropriate vector. The eukaryotic host cell does not produce protein bodies in the absence of the fusion protein. Methods for preparing and using the RPBLAs and the fusion protein are also disclosed, as are nucleic acid molecules that encode the fusion proteins.

Abstract: The instant disclosure describes the application of genetic engineering techniques to produce cellulase in plants. Cellulase coding sequences operably linked to promoters active in plants may be transformed into the nuclear genome and/or the plastid genome of a plant. As cellulases may be toxic to plants, chemically-inducible or wound-inducible promoters may be employed. Additionally, the expressed cellulases may be targeted to vacuoles or other cellular organelles.

Abstract: Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.

Abstract: The invention provides novel stress tolerant transgenic plants which express trehalose 6-phosphate synthase and trehalose-6-phosphate phosphatase in their plastids.

Abstract: The present invention relates to a method to confer resistance or tolerance to more than one virus from the group consisting of furovirus, potyvirus, tospovirus, and cucomovirus, using sense and antisense RNA fragments of a sequence from their genomes. The sense and antisense RNA fragments are capable of pairing and forming a double-stranded RNA molecule, thereby reducing expression of the viral genome.

Abstract: The invention provides novel transgenic plants which express trehalose biosynthetic genes, e.g., under control of an inducible promoter, which are developmentally normal, together with methods for improving stress tolerance in said plants, methods of improving food quality, and other methods of making and using the plants of the invention. The invention also provides nucleotide sequences encoded novel trehalose biosynthetic enzymes.

Abstract: The invention provides novel transgenic plants which express trehalose biosynthetic genes, e.g., under control of an inducible promoter, which are developmentally normal, together with methods for improving stress tolerance in said plants, methods of improving food quality, and other methods of making and using the plants of the invention. The invention also provides nucleotide sequences encoded novel trehalose biosynthetic enzymes.

Abstract: Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobulins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.

Abstract: The invention provides novel transgenic plants which express trehalose biosynthetic genes, e.g., under control of an inducible promoter, which are developmentally normal, together with methods for improving stress tolerance in said plants, methods of improving food quality, and other methods of making and using the plants of the invention. The invention also provides nucleotide sequences encoded novel trehalose biosynthetic enzymes.

Abstract: A novel promoter isolated from the 5′ flanking region upstream of the coding sequence of the Arabidopsis plastid clpP gene is described. Also described are a novel method for utilizing protein-coding regions of plastid genes to isolate intervening regulatory sequences and a novel method for improving plastid transformation efficiency using exogenous plastid promoters that differ in nucleotide sequence from native plastid promoters.