Abstract: Provided herein are prime editing methods and compositions for treatment of genetic disorders such as Friedreich ataxia.

Abstract: Disclosed are a method for chip packaging having a high-density connection layer and a chip packaging structure, the method comprising S1: preparing a high-density connection layer having multilayered metal wiring layer; S2: preparing a substrate intermediate, and attaching thereto the high-density connection layer; S3: embedding the high-density connection layer into the substrate and preparing a first type pads connected with the multilayered metal wiring layer and a second type pads connected with a wiring layer of the substrate intermediate; S4: inversely mounting the chip on the substrate surface, the first type chip bumps being connected to the first type pads, and the second type chip bumps being connected to the second type pads. Part of the wiring layer inside the substrate is replaced with single-sided or double-sided high-density connection layer to reduce the number of layers and thickness of the substrate, facilitating layout and wiring and improving integration and performance.

Abstract: A catheter is disclosed comprising a catheter shaft including a proximal end and a distal end. A flexible framework can be connected to the distal end of the catheter shaft, wherein the flexible framework includes a plurality of heating electrodes and a temperature sensor. The plurality of heating electrodes can be configured to be heated to a first temperature, the first temperature being lower than which radio frequency ablation is performed. The plurality of heating electrodes can be configured to be heated to a second temperature, the second temperature being a temperature at which radio frequency ablation is performed.

Abstract: A method for data storage includes receiving, by a storage device, a read request for data, the read request being associated with a request size, determining that a first cache area associated with a first portion of the data is in a first portion of a cache, the first cache area having a first size that is smaller than the request size, determining that a second cache area associated with a second portion of the data is in a second portion of the cache, the second cache area having a second size that is smaller than the request size and differently sized than the first size, and based on the read request, reading the first portion of the data from the first cache area and reading the second portion of the data from the second cache area.

Abstract: A catheter configured to deliver therapeutic energy to a tissue is disclosed. The catheter comprises an elongate shaft extending along a shaft longitudinal axis. The catheter further comprises a flexible tip assembly coupled to a distal end of the elongate shaft, the flexible tip assembly comprising at least three mapping electrodes equally spaced about a circumference of the flexible tip, a flexible ablation electrode, and a plurality of irrigation ports. The catheter further comprises an irrigation lumen extending through the elongate shaft and into an interior of the flexible tip assembly, wherein the irrigation lumen is configured to direct irrigation fluid to the plurality of irrigation ports.

Abstract: Disclosed are a method for chip packaging having a high-density connection layer and a chip packaging structure, the method comprising S1: preparing a high-density connection layer having multilayered metal wiring layer; S2: preparing a substrate intermediate, and attaching thereto the high-density connection layer; S3: embedding the high-density connection layer into the substrate and preparing a first type pads connected with the multilayered metal wiring layer and a second type pads connected with a wiring layer of the substrate intermediate; S4: inversely mounting the chip on the substrate surface, the first type chip bumps being connected to the first type pads, and the second type chip bumps being connected to the second type pads. Part of the wiring layer inside the substrate is replaced with single-sided or double-sided high-density connection layer to reduce the number of layers and thickness of the substrate, facilitating layout and wiring and improving integration and performance.

Abstract: The present invention provides an improved percutaneous dilation tracheostomy device. The device is configured to include all of the required components to perform a percutaneous tracheotomy. The device includes a retractable needle and an extendable j-wire rather than having separate components as in typical percutaneous tracheostomy devices. The device includes a dilator section to expand the diameter of a patient's stoma. The device is further configured to allow an operator to perform a bubble test to alert the user that the tube is in the trachea. In addition, the device is generally more compact than typical emergency tracheostomy devices.

Abstract: A catheter is disclosed comprising a catheter shaft including a proximal end and a distal end. A flexible framework can be connected to the distal end of the catheter shaft, wherein the flexible framework includes a plurality of heating electrodes and a temperature sensor. The plurality of heating electrodes can be configured to be heated to a first temperature, the first temperature being lower than which radio frequency ablation is performed. The plurality of heating electrodes can be configured to be heated to a second temperature, the second temperature being a temperature at which radio frequency ablation is performed.

Abstract: A lighter housing for a lighter unit includes a bottom housing and a top housing. The bottom housing defines an interior chamber for accommodating the lighter unit. The top housing is above the lighter unit and connected to the bottom housing. A plurality of convex elements are periodically arranged on and extending from an exterior face of the lighter housing, and a plurality of concave cells are defined among the plurality of the convex elements.

Abstract: A catheter configured to deliver therapeutic energy to a tissue is disclosed. The catheter comprises an elongate shaft extending along a shaft longitudinal axis. The catheter further comprises a flexible tip assembly coupled to a distal end of the elongate shaft, the flexible tip assembly comprising at least three mapping electrodes equally spaced about a circumference of the flexible tip, a flexible ablation electrode, and a plurality of irrigation ports. The catheter further comprises an irrigation lumen extending through the elongate shaft and into an interior of the flexible tip assembly, wherein the irrigation lumen is configured to direct irrigation fluid to the plurality of irrigation ports.

Abstract: A quantum dot structure is provided, the quantum dot structure comprising: a nanocrystalline core from a first semiconductor material, a nanocrystalline shell from a second semiconductor material on the nanocrystalline core, at least one encapsulation layer on the nanocrystalline shell, wherein functional groups are present within the at least one encapsulation layer and/or on the surface of the at least one encapsulation layer facing away from the nanocrystalline shell, the functional groups being able to chemically react in a reversible manner. Further, a method for producing a quantum dot structure and a light emitting device are provided.

Abstract: A method for data storage includes receiving, by a storage device, a read request for data, the read request being associated with a request size, determining that a first cache area associated with a first portion of the data is in a first portion of a cache, the first cache area having a first size that is smaller than the request size, determining that a second cache area associated with a second portion of the data is in a second portion of the cache, the second cache area having a second size that is smaller than the request size and differently sized than the first size, and based on the read request, reading the first portion of the data from the first cache area and reading the second portion of the data from the second cache area.

Abstract: Systems and methods for managing a storage device are disclosed. A first amount of a first storage device is associated to a first client. A first request associated with first data is detected, and a second request associated with the first data is also detected. A log for the first request and the second request is maintained for the first client. A trigger condition is identified, and based on identifying the trigger condition, a value is computed based on the log. A second amount of the first storage device is determined based on the value, and the second amount is associated to the first client.

Abstract: The present invention provides an improved percutaneous dilation tracheostomy device. The device is configured to include all of the required components to perform a percutaneous tracheotomy. The device includes a retractable needle and an extendable j-wire rather than having separate components as in typical percutaneous tracheostomy devices. The device includes a dilator section to expand the diameter of a patient's stoma. The device is further configured to allow an operator to perform a bubble test to alert the user that the tube is in the trachea. In addition, the device is generally more compact than typical emergency tracheostomy devices.

Abstract: The present invention provides an improved percutaneous dilation tracheostomy device. The device is configured to include all of the required components to perform a percutaneous tracheotomy. The device includes a retractable needle and an extendable j-wire rather than having separate components as in typical percutaneous tracheostomy devices. The device includes a dilator section to expand the diameter of a patient's stoma. The device is further configured to allow an operator to perform a bubble test to alert the user that the tube is in the trachea. In addition, the device is generally more compact than typical emergency tracheostomy devices.