Abstract: Disclosed are methods for transducing cells, e.g., NK cells, comprising: contacting the cells with a transduction composition, e.g., a virus particle, and a TBK1/IKK? inhibitor, e.g., the TBK1/IKK? inhibitor can comprise a substituted N-(3-((2-((3-(aminomethyl)-phenyl)amino)-5-methylpyrimidin-4-yl)amino)propyl)acetamide analogue; thereby transducing the cells. The virus particle can comprises a lentiviral vector. The present disclosure also relates to kits comprising: a TBK1/IKK? inhibitor; and instructions for transducing NK cells; wherein the TBK1/IKK? inhibitor comprises a substituted N-(3-((2-((3-(aminomethyl)-phenyl)amino)-5-methylpyrimidin-4-yl)amino)propyl)acetamide analogue. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Abstract: The present application provides chimeric antigen receptors (CARs) comprising an anchoring domain, such as a PDZ binding motif, which binds to a cell polarity protein. Also provided are poly nucleotides encoding the CARs, vectors, and cell compositions comprising the same. Pharmaceutical compositions comprising the polypeptides, polynucleotides, vectors, or cells of the present disclosure, and their uses in treating a disease in a subject are also provided.