Abstract: A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Abstract: A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Abstract: Means and methods for generating binding peptide associated with a suitable core region are disclosed, the resulting proteinaceous molecule and uses thereof. A solution to the problems associated with the use of binding molecules over their entire range of use. Binding molecules can be designed to accommodate extreme conditions of use such as extreme temperatures or pH. Alternatively, binding molecules can be designed to respond to very subtle changes in the environment.

Abstract: Proteinaceous molecules involved in molecular binding are disclosed. The binding molecules have a common structural core element that is provided with a desired affinity region. The core element and the desired affinity region can be adapted to bind to a wide range of molecules for a desired purpose. A method for identifying a binding molecule with an altered binding property and producing a nucleic acid for the identified binding molecule are also disclosed.

Abstract: Nematode-inducible promoter sequences are disclosed. These sequences can be used for expressing DNA sequences in plant cells. Chimeric DNA constructs comprising the nematode-inducible promoter sequences operably linked to DNA to be expressed in plant cells are also disclosed. Plants comprising the chimeric DNA are provided.

Abstract: An isolated DNA fragment that promotes root knot or cyst nematode indcuible transcription of a coding sequence downstream of and operably linked to the fragment in at least an Arabidopsis thaliana plant and a chimeric DNA sequence including the DNA fragment. Also, methods for the use of the DNA fragment.

Abstract: A method for obtaining plants with reduced susceptibility to invasion by a plant parasitic nematode, and plants having reduced susceptibility to invasion by a plant parasitic nematode obtained, for example, with such method.

Abstract: The invention provides a method for infecting a root of a plant of the genus Arabidopsis with a plant parasitic nematode under monoxenic conditions, comprising the steps of: (a) contacting the root with a plant parasitic nematode in the presence of a gelling matrix dissolved in a nutrient medium that is substantially free of nematode inhibiting substances, and (b) allowing the nematode to infect the root by forming a feeding structure.

Abstract: This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.

Abstract: This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.

Abstract: The present invention provides plants with a modified taste, solids content and/or texture. The invention also provides methods of obtaining such plants via transformation with DNA constructs containing genes encoding enzymes capable of degrading plant polysaccharides and optionally additional genes encoding enzymes which are capable of further modifying the degradation products resulting from the first degradation step.

Abstract: This invention provides for a method to produce excreted and correctly processed heterologous protein-material in a plant host. The method comprises the step of transforming a plant host using a recombinant polynucleotide, which comprises the DNA sequences encoding the fully processed heterologous protein material, directly preceded by a DNA sequence coding for a plant signal peptide, and regulatory sequences necessary for the plant host to express the heterologous gene construct, resulting in excretion of the heterologous protein from the cell and correct cleavage of the N-terminal signal peptide, so that the mature heterologous protein produced by the plant host is identical to the corresponding protein produced in its authentic host.