Patents by Inventor Peter J. Belshaw
Peter J. Belshaw has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8563777Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.Type: GrantFiled: June 8, 2011Date of Patent: October 22, 2013Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the University of IllinoisInventors: Lloyd M. Smith, Michael R. Shortreed, Brian L. Frey, Margaret F. Phillips, Joshua J. Coon, Shane M. Lamos, Casey J. Krusemark, Peter J. Belshaw, Madhusudan Patel, Neil L. Kelleher
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Publication number: 20120022230Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.Type: ApplicationFiled: June 8, 2011Publication date: January 26, 2012Applicant: Wisconsin Alumni Research FoundationInventors: Lloyd M. SMITH, MICHAEL R. SHORTREED, BRIAN L. FREY, MARGARET F. PHILLIPS, JOSHUA J. COON, SHANE M. LAMOS, CASEY J. KRUSEMARK, PETER J. BELSHAW, MADHUSUDAN PATEL, NEIL L. KELLEHER
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Patent number: 8008005Abstract: A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will spontaneously hybridize together to form the desired DNA construct. This method makes possible the remote assembly of DNA sequence, through a process analogous to facsimile transmission of documents, since the information on DNA to be made can be transmitted remotely to an instrument which can then synthesize any needed DNA sequence from the information.Type: GrantFiled: January 24, 2007Date of Patent: August 30, 2011Assignee: Wisconsin Alumni Research FoundationInventors: Peter J. Belshaw, Michael J. Sussman, Francesco Cerrina, Shane T. Flickinger
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Patent number: 7982070Abstract: Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic kit facilitating relative quantification and providing tangible evidence for the existence of specific functional groups. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules, such as biological samples. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. Advantageously, the labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications.Type: GrantFiled: March 21, 2007Date of Patent: July 19, 2011Assignee: Wisconsin Alumni Research FoundationInventors: Lloyd M. Smith, Michael R. Shortreed, Brian L. Frey, Margaret F. Phillips, Joshua J. Coon, Shane M. Lamos, Casey J. Krusemark, Peter J. Belshaw, Madhusudan Patel, Neil L. Kelleher
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Publication number: 20090188793Abstract: A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The complement of each segment is also made in the microarray. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will hybridize to form duplexes. The duplexes are then separated using a DNA binding agent which hinds to improperly formed DNA helixes to remove errors form the set of DNA molecules. The segments can then be hybridized to each other to assemble the larger target DNA sequence.Type: ApplicationFiled: October 23, 2007Publication date: July 30, 2009Inventors: Michael R. Sussman, Francesco Cerrina, Peter J. Belshaw, James H. Kaysen, Kathryn Richmond
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Patent number: 7303872Abstract: A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The complement of each segment is also made in the microarray. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will hybridize to form duplexes. The duplexes are then separated using a DNA binding agent which binds to improperly formed DNA helixes to remove errors from the set of DNA molecules. The segments can then be hybridized to each other to assemble the larger target DNA sequence.Type: GrantFiled: February 28, 2003Date of Patent: December 4, 2007Assignee: Wisconsin Alumni Research FoundationInventors: Michael R. Sussman, Francesco Cerrina, Peter J. Belshaw, James H. Kaysen, Kathryn Richmond
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Patent number: 7183406Abstract: A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will spontaneously hybridize together to form the desired DNA construct. This method makes possible the remote assembly of DNA sequence, through a process analogous to facsimile transmission of documents, since the information on DNA to be made can be transmitted remotely to an instrument which can then synthesize any needed DNA sequence from the information.Type: GrantFiled: May 20, 2002Date of Patent: February 27, 2007Assignee: Wisconsin Alumni Research FoundationInventors: Peter J Belshaw, Michael J. Sussman, Francesco Cerrina, Shane T. Flickinger
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Patent number: 6982082Abstract: This invention is directed to a modified cyclosporin A and to a modified, genetically engineered version of its receptor, cyclophilin. This invention is further directed to a method for treating host versus graft disease following blood marrow transplantation by transfecting stem cells so that after introduction into a patient the stem cells will express the modified cyclophilin, and, as necessary, administer the modified cyclosporin A to the patient.Type: GrantFiled: August 27, 1997Date of Patent: January 3, 2006Assignees: President and Fellows of Harvard College, Board of Trustees of the Leland Stanford Junior UniversityInventors: Stuart L. Schreiber, Peter J. Belshaw, Gerald Crabtree
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Publication number: 20040132029Abstract: A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The complement of each segment is also made in the microarray. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will hybridize to form duplexes. The duplexes are then separated using a DNA binding agent which binds to improperly formed DNA helixes to remove errors from the set of DNA molecules. The segments can then be hybridized to each other to assemble the larger target DNA sequence.Type: ApplicationFiled: February 28, 2003Publication date: July 8, 2004Inventors: Michael R. Sussman, Francesco Cerrina, Peter J. Belshaw, James H. Kaysen, Kathryn Richmond
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Publication number: 20030068633Abstract: A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will spontaneously hybridize together to form the desired DNA construct. This method makes possible the remote assembly of DNA sequence, through a process analogous to facsimile transmission of documents, since the information on DNA to be made can be transmitted remotely to an instrument which can then synthesize any needed DNA sequence from the information.Type: ApplicationFiled: May 20, 2002Publication date: April 10, 2003Inventors: Peter J. Belshaw, Michael J. Sussman, Francesco Cerrina