Patents by Inventor Peter Jan Punt
Peter Jan Punt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11066681Abstract: The invention relates to a method to increase production of itaconic acid in a micro-organism by inhibiting the expression or functioning of the enzyme itaconyl-CoA transferase, itaconyl-CoA hydratase (citramalyl-CoA hydrolyase; EC 4.2.1.56) and/or citramalyl-CoA lyase. Also embodied are micro-organisms, preferably Aspergillus terreus or Aspergillus niger, in which said enzyme is inhibited.Type: GrantFiled: August 25, 2017Date of Patent: July 20, 2021Assignee: LESAFFRE ET COMPAGNIEInventor: Peter Jan Punt
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Publication number: 20190177751Abstract: The invention relates to a method to increase production of itaconic acid in a micro-organism by inhibiting the expression or functioning of the enzyme itaconyl-CoA transferase, itaconyl-CoA hydratase (citramalyl-CoA hydrolyase; EC 4.2.1.56) and/or citramalyl-CoA lyase. Also embodied are micro-organisms, preferably Aspergillus s terreus or Aspergillus niger, in which said enzyme is inhibited.Type: ApplicationFiled: August 25, 2017Publication date: June 13, 2019Inventor: Peter Jan PUNT
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Publication number: 20180273986Abstract: The invention relates to the use of a cytosolic citric acid synthase for the heterologous production of citrate outside the mitochondrion of a micro-organism or algae, wherein the protein is selected from A. niger An08g10920, An01g09940, An09g03570 ,or an ortholog of these genes. Such production is achieved by introducing the nucleic acid encoding such a protein into a suitable host cell. Preferably the protein is A. niger An08g10920, An01g09940, An09g03570 or an ortholog thereof, more particularly, wherein such an ortholog is chosen from the group of proteins listed in FIG. 9 and proteins having a percentage identity of 70%, more preferably 75%, more preferably 80%, more preferably 85%, more preferably 90%, more preferably 95%, more preferably 98%, more preferably 99% with An08g10920, An01g09940 or An09g03570.Type: ApplicationFiled: December 20, 2017Publication date: September 27, 2018Applicant: DUTCH DNA BIOTECH B.V.Inventors: Peter Jan PUNT, An LI, Martinus Petrus Maria CASPERS
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Patent number: 9885066Abstract: The invention relates to the use of a cytosolic citric acid synthase for the heterologous production of citrate outside the mitochondrion of a micro-organism or algae, wherein the protein is selected from A. niger An08g10920, An01g09940, An09g03570, or an ortholog of these genes. Such production is achieved by introducing the nucleic acid encoding such a protein into a suitable host cell. Preferably the protein is A. niger An08g10920, An01g09940, An09g03570 or an ortholog thereof, more particularly, wherein such an ortholog is chosen from the group of proteins listed in FIG. 9 and proteins having a percentage identity of 70%, more preferably 75%, more preferably 80%, more preferably 85%, more preferably 90%, more preferably 95%, more preferably 98%, more preferably 99% with An08g10920, An01g09940 or An09g03570.Type: GrantFiled: May 2, 2014Date of Patent: February 6, 2018Assignee: DUTCH DNA BIOTECH B.V.Inventors: Peter Jan Punt, An Li, Martinus Petrus Maria Caspers
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Patent number: 9359612Abstract: The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of Aspergillus terreus or homologues thereof. Overexpression of the protein enhances the production of itaconic acid in micro-organisms.Type: GrantFiled: January 6, 2014Date of Patent: June 7, 2016Assignee: DUTCH DNA BIOTECH B.V.Inventors: Maria Johanna Van Der Werf, Peter Jan Punt
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Patent number: 9290772Abstract: The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbors other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.Type: GrantFiled: December 20, 2013Date of Patent: March 22, 2016Assignee: DUTCH DNA BIOTECH B. V.Inventors: Peter Jan Punt, Maria Johanna Van Der Werf
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Publication number: 20160068873Abstract: The invention relates to the use of a cytosolic citric acid synthase for the heterologous production of citrate outside the mitochondrion of a micro-organism or algae, wherein the protein is selected from A. niger An08g10920, An01g09940, An09g03570, or an ortholog of these genes. Such production is achieved by introducing the nucleic acid encoding such a protein into a suitable host cell. Preferably the protein is A. niger An08g10920, An01g09940, An09g03570 or an ortholog thereof, more particularly, wherein such an ortholog is chosen from the group of proteins listed in FIG. 9 and proteins having a percentage identity of 70%, more preferably 75%, more preferably 80%, more preferably 85%, more preferably 90%, more preferably 95%, more preferably 98%, more preferably 99% with An08g10920, An01g09940 or An09g03570.Type: ApplicationFiled: May 2, 2014Publication date: March 10, 2016Applicant: Dutch DNA Biotech B.V.Inventors: Peter Jan PUNT, An LI, Martinus Petrus Maria CASPERS
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Patent number: 9255284Abstract: Methods to digest carbohydrates, especially lignocelluloses and hemicelluloses, using fungal proteins previously not recognized as having this activity are described.Type: GrantFiled: April 15, 2014Date of Patent: February 9, 2016Assignee: NEDERLANDSE ORGANISATIE VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK TNOInventor: Peter Jan Punt
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Patent number: 8871493Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: GrantFiled: September 17, 2012Date of Patent: October 28, 2014Assignee: Dyadic International (USA), Inc.Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Marie Johanna Van Zeijl
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Publication number: 20140315260Abstract: Methods to digest carbohydrates, especially lignocelluloses and hemicelluloses, using fungal proteins previously not recognized as having this activity are described.Type: ApplicationFiled: April 15, 2014Publication date: October 23, 2014Applicant: NEDERLANDSE ORGANISATIE VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK TNOInventor: Peter Jan PUNT
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Publication number: 20140199754Abstract: The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of Aspergillus terreus or homologues thereof. Overexpression of the protein enhances the production of itaconic acid in micro-organisms.Type: ApplicationFiled: January 6, 2014Publication date: July 17, 2014Applicant: Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNOInventors: Maria Johanna VAN DER WERF, Peter Jan PUNT
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Publication number: 20140193885Abstract: The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbours other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.Type: ApplicationFiled: December 20, 2013Publication date: July 10, 2014Applicant: NEDERLANDSE ORGANISATIE VOOR TOEGEPAST- NATUURWETENSCHAPPELIJK ONDERZOEKInventors: Peter Jan PUNT, Maria Johanna VAN DER WERF
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Patent number: 8735100Abstract: Methods to digest carbohydrates, especially lignocelluloses and hemicelluloses, using fungal proteins previously not recognized as having this activity are described.Type: GrantFiled: March 17, 2010Date of Patent: May 27, 2014Assignee: Nederlandse Organisatie Voor Toegepast -Natuurwetenschappelijk Onderzoek TNOInventor: Peter Jan Punt
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Publication number: 20140127788Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: ApplicationFiled: September 17, 2012Publication date: May 8, 2014Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
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Patent number: 8679801Abstract: The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbors other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.Type: GrantFiled: February 16, 2009Date of Patent: March 25, 2014Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek TNOInventors: Peter Jan Punt, Maria Johanna Van Der Werf
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Patent number: 8642298Abstract: The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of Aspergillus terreus or homologues thereof. Overexpression of the protein enhances the production of itaconic acid in microorganisms.Type: GrantFiled: March 4, 2009Date of Patent: February 4, 2014Assignee: Nederlandse Organisatie Voor Toegepast—Natuurwetenschappelijk Onderzoek TnoInventors: Maria Johanna Van Der Werf, Peter Jan Punt
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Publication number: 20130143271Abstract: The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.Type: ApplicationFiled: March 14, 2011Publication date: June 6, 2013Inventors: Mark Aaron Emalfarb, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
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Patent number: 8440436Abstract: The invention relates to the production of itaconic acid in micro-organisms by introducting into a suitable host cell a gene coding for the enzyme cis-aconitic acid decarboxylase, preferably derived from A. terreus. Further part of the invention are host cells, preferably from A. niger, provided with the above mentioned genes.Type: GrantFiled: July 21, 2008Date of Patent: May 14, 2013Assignee: Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNOInventors: Maria Johanna Van Der Werf, Martinus Petrus Maria Caspers, Nicole Van Luijk, Peter Jan Punt
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Patent number: 8268585Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: GrantFiled: March 13, 2008Date of Patent: September 18, 2012Assignee: Dyadic International (USA), Inc.Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
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Publication number: 20120088273Abstract: The invention relates to a lignocellulose active protein comprising a DUF1996 domain and proteins that additionally comprise a CBM1 domain. Also included in the invention are proteins with a new domain, characterized by the consensus sequence [GA]-[ST]-[IV]-[ILV]-W-[DS]-G-[RIFS]-F-[ND]-[DS]-X (residues 1-12 of SEQ ID NOS:6-7). The invention relates to a lignocellulose active protein comprising a DUF1996 domain and proteins that additionally comprise a CBM1 domain.Type: ApplicationFiled: March 17, 2010Publication date: April 12, 2012Applicant: Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek TnoInventor: Peter Jan Punt