Abstract: The invention relates to the detection of the cofactor reduced nicotinamide adenine dinucleotide phosphate (NADPH). Provided is a sensor molecule for the resonance energy transfer (RET)-based detection of NADPH, the sensor comprising a segment A connected via a linker to a segment B, wherein each of segment A and segment B comprises a member of a RET pair comprising a donor moiety and an acceptor moiety, further characterized in that (i) segment A comprises a binding protein (BP) for NADPH, the BP being dihydrofolate reductase (DHFR; EC 1.5.1.3) or a functional homolog, fragment, derivative or variant thereof, showing the desired NADPH binding properties, and wherein the BP comprises a heterologous protein domain inserted at or replacing at least part of the region corresponding to positions (20) to (27) of E.

Abstract: A feeding device for feeding stacks of standing flat-folded cartons to a receiving station, comprising a conveyor track having a flat bearing plane adapted to support the stacks, where the conveyor track comprises at least two rows of teeth where each row of teeth comprises a plurality of spaced apart teeth adapted to support the stacks of flat-folded cartons, where the teeth can be positioned in either an erected position or in a folded position, and where the feeding device comprises a guide track arranged along the bearing plane adapted to hold the teeth in the selected position, and where the guide track ends at an end region of the conveyor track, such that the teeth are unsupported at the end region. The advantage of the invention is that stacks of flat-folded cartons can be merged continuously at an outfeed region.

Abstract: A divider for dividing a pack of flat-folded cartons into a plurality of stacks of flat-folded cartons, the divider comprising a magazine adapted to hold the carton pack, where the magazine includes a stop member, and where the divider includes a pusher member and a holder foot, where the magazine is arranged in an inclined position, where the pusher member is adapted to compress the carton pack between the pusher member and the stop member, where the holder foot is adapted to bear on the upper side of the compressed carton pack, and where the pusher member is adapted to release the pressure on the carton pack when the holder foot bears on the upper side of the carton pack, thereby creating a low density area adjacent a first carton stack.

Abstract: The invention relates to the detection of the cofactor reduced nicotinamide adenine dinucleotide phosphate (NADPH). Provided is a sensor molecule for the resonance energy transfer (RET)-based detection of NADPH, the sensor comprising a segment A connected via a linker to a segment B, wherein each of segment A and segment B comprises a member of a RET pair comprising a donor moiety and an acceptor moiety, further characterized in that (i) segment A comprises a binding protein (BP) for NADPH, the BP being dihydrofolate reductase (DHFR; EC 1.5.1.3) or a functional homolog, fragment, derivative or variant thereof, showing the desired NADPH binding properties, and wherein the BP comprises a heterologous protein domain inserted at or replacing at least part of the region corresponding to positions (20) to (27) of E.

Abstract: A divider for dividing a pack of flat-folded cartons into a plurality of stacks of flat-folded cartons, the divider comprising a magazine adapted to hold the carton pack, where the magazine includes a stop member, and where the divider includes a pusher member and a holder foot, where the magazine is arranged in an inclined position, where the pusher member is adapted to compress the carton pack between the pusher member and the stop member, where the holder foot is adapted to bear on the upper side of the compressed carton pack, and where the pusher member is adapted to release the pressure on the carton pack when the holder foot bears on the upper side of the carton pack, thereby creating a low density area adjacent a first carton stack.

Abstract: Gripping device adapted for gripping a stack of flat-folded cartons, where the gripping device comprises a first gripping jaw and a second gripping jaw arranged adjacent each other and enclosing a holding space, where the gripping device is provided with a first open state in which the distance between the gripping jaws is greater than the height of a flat-folded carton, and where the gripping device is provided with a second closed state in which the distance between the gripping jaws is less than the height of a flat-folded carton, and that the gripping device is adapted to displace one side of the stack of flat-folded cartons in a longitudinal direction, such that each flat-folded carton of the stack of flat-folded cartons will be held in an angled state by the gripping device. The advantage of the invention is that a stack of flat-folded cartons can be held in a secure way, where the height of the stack is reduced when the stack is held.

Abstract: Gripping device adapted for gripping a stack of flat-folded cartons, where the gripping device comprises a first gripping jaw and a second gripping jaw arranged adjacent each other and enclosing a holding space, where the gripping device is provided with a first open state in which the distance between the gripping jaws is greater than the height of a flat-folded carton, and where the gripping device is provided with a second closed state in which the distance between the gripping jaws is less than the height of a flat-folded carton, and that the gripping device is adapted to displace one side of the stack of flat-folded cartons in a longitudinal direction, such that each flat-folded carton of the stack of flat-folded cartons will be held in an angled state by the gripping device. The advantage of the invention is that a stack of flat-folded cartons can be held in a secure way, where the height of the stack is reduced when the stack is held.

Abstract: The invention relates to the in vitro and in cellulo detection of the cofactors nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Provided is a sensor molecule for fluorescence- or luminescence-based detection of a nicotinamide adenine dinucleotide analyte, in particular for detecting the concentrations of NAD+, NADP+ and/or the ratios of the concentrations of NAD+/NADH and NADP+/NADPH, the sensor comprising (i) a binding protein (BP) for the nicotinamide adenine dinucleotide analyte, the BP being derived from sepiapterin reductase (SPR; EC 1.1.1.153) (ii) an SPR ligand (SPR-L) capable of intramolecular binding to said BP in the presence of the oxidized form of said analyte; and (iii) at least one fluorophore.

Abstract: Inhibitors of sepiapterin reductase and uses of sepiapterin reductase inhibitors in analgesia, treatment of acute and chronic pain, anti-inflammation, and immune cell regulation are disclosed.

Abstract: The invention relates to the field of in vitro detection methods using luminescence. Provided is a sensor molecule for detecting an analyte of interest in a sample using bioluminescence resonance energy transfer (BRET), the sensor molecule comprising a proteinaceous moiety tethered to a synthetic regulatory molecule. Also provided is an analytical device comprising a sensor and methods using the sensor molecule.

Abstract: The invention relates to the in vitro and in cellulo detection of the cofactors nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Provided is a sensor molecule for fluorescence or luminescence-based detection of a nicotinamide adenine dinucleotide analyte, in particular for detecting the concentrations of NAD+, NADP? and/or the ratios of the concentrations of NAD?/NAD H and NADP?/NADPH, the sensor comprising (i) a binding protein (BP) for the nicotinamide adenine dinucleotide analyte, the BP being derived from sepiapterin reductase (SPR; EC 1.1.1.153) (ii) a SPR ligand (SPR-L) capable of intramolecular binding to said BP in the presence of the oxidized form of said analyte; and (iii) at least one fluorophore.

Abstract: Inhibitors of sepiapterin reductase and uses of sepiapterin reductase inhibitors in analgesia, treatment of acute and chronic pain, anti-inflammation, and immune cell regulation are disclosed.

Abstract: The invention relates to the field of in vitro detection methods using luminescence. Provided is a sensor molecule for detecting an analyte of interest in a sample using bioluminescence resonance energy transfer (BRET), the sensor molecule comprising a proteinaceous moiety tethered to a synthetic regulatory molecule. Also provided is an analytical device comprising a sensor and methods using the sensor molecule.

Abstract: Embodiments include a method or a device for a cartoning machine for transferring a leaflet from a first transportation device to a leaflet holder in a second transportation device. Embodiments include a transferring device which is arranged to straighten up and position the leaflets in such a way that the leaflet holders can take hold of the leaflets. The transferring device has a base surface with one or more carriers placed in a line at right angels to the direction of transportation, so that the leaflet, as a result of being able to slide against the base surface, is pressed back against the carriers as a result of air resistance friction against an adjacent cover plate and/or centripetal forces. The base surface of the transferring device is preferably provided with several lines of carriers which are adjusted to match the distance between the leaflet holders on the second transportation device.

Abstract: The invention relates to a method and a device for a cartoning machine for transferring an leaflet from a first transportation device to an leaflet holder comprised in a second transportation device. The machine comprises a transferring device which is arranged to straighten up and position the leaflets in such a way that the leaflet holders can take hold of the leaflets without them ending up askew. The transferring device has a base surface with one or more carriers placed in a line at right angles to the direction of transportation, so that the leaflet, as a result of being able to slide against the base surface, is pressed back against the carriers as a result of air resistance, friction against an adjacent cover plate and/or centripetal forces. The base surface of the transferring device is preferably provided with several lines of carriers which are adjusted to match the distance between the leaflet holders on the second transportation device.