Abstract: The disclosure relates to methods and compositions for regulating expression of DUX4. In some aspects, methods described by the disclosure are useful for treating a disease associated with aberrant DUX4 expression (e.g., facioscapulohumeral muscular dystrophy).

Abstract: The present invention relates to methods, devices and systems for associating consumable data with an assay consumable used in a biological assay. Provided are assay systems and associated consumables, wherein the assay system adjusts one or more steps of an assay protocol based on consumable data specific for that consumable. Various types of consumable data are described, as well as methods of using such data in the conduct of an assay by an assay system. The present invention also relates to consumables (e.g., kits and reagent containers), software, data deployable bundles, computer-readable media, loading carts, instruments, systems, and methods, for performing automated biological assays.

Abstract: A gondola railroad car partially formed using members of high strength steel, that is lighter than conventional gondola railroad cars, that can carry more weight than conventional gondola railroad cars, that is less prone to damage than conventional gondola railroad cars, and that has a longer lifespan than convention gondola railroad cars. In various such embodiments, the floor and the side walls of the gondola railroad car are partially formed from ultra-high strength steel members.

Abstract: A large plate tilt railroad car including a plate support assembly having a plurality of spaced-apart plate supporters including a plurality of plate holders simultaneously movable from loading positions in which one or more large steel plates can be loaded on the railroad car to transit positions in which one or more large steel plates can be transported using the railroad car.

Abstract: Various embodiments provide a hopper railroad car top hatch cover assembly including a hatch cover and a hatch cover securer configured to secure the hatch cover in a closed position engaging a coaming of the hopper railroad car. Various embodiments provide a hopper railroad car having a top hatch cover assembly including a hatch cover and a hatch cover securer configured to secure the hatch cover in a closed position engaging a coaming of the hopper railroad car.

Abstract: A railroad hopper car having a single cargo-receiving hopper extending over substantially the length of a car body having a cargo discharge portion occupying most of the available distance between the wheeled trucks supporting opposite ends of the car body. The car has no pair of slope sheets separating longitudinally-adjacent hoppers and defining unusable space beneath the slope sheets, and so the overall length of such a hopper car capable of carrying a desired volume of cargo can be less than that of one with multiple separate hoppers. Cargo discharge openings are arrayed along the length of the cargo discharge portion, and a unitary multi-gate assembly is supported within the cargo discharge portion of the car body so as to be movable longitudinally of the car body to open or close all of the cargo discharge openings simultaneously.

Abstract: A large plate tilt railroad car including a plate support assembly having a plurality of spaced-apart plate supporters including a plurality of plate holders simultaneously movable from loading positions in which one or more large steel plates can be loaded on the railroad car to transit positions in which one or more large steel plates can be transported using the railroad car.

Abstract: The disclosure relates to methods and compositions for regulating expression of DUX4. In some aspects, methods described by the disclosure are useful for treating a disease associated with aberrant DUX4 expression (e.g., facioscapulohumeral muscular dystrophy. FSHD).

Abstract: Various embodiments provide a hopper railroad car top hatch cover assembly including a hatch cover and a hatch cover securer configured to secure the hatch cover in a closed position engaging a coaming of the hopper railroad car. Various embodiments provide a hopper railroad car having a top hatch cover assembly including a hatch cover and a hatch cover securer configured to secure the hatch cover in a closed position engaging a coaming of the hopper railroad car.

Abstract: A railroad hopper car having a single cargo-receiving hopper extending over substantially the length of a car body having a cargo discharge portion occupying most of the available distance between the wheeled trucks supporting opposite ends of the car body. The car has no pair of slope sheets separating longitudinally-adjacent hoppers and defining unusable space beneath the slope sheets, and so the overall length of such a hopper car capable of carrying a desired volume of cargo can be less than that of one with multiple separate hoppers. Cargo discharge openings are arrayed along the length of the cargo discharge portion, and a unitary multi-gate assembly is supported within the cargo discharge portion of the car body so as to be movable longitudinally of the car body to open or close all of the cargo discharge openings simultaneously.

Abstract: The disclosure relates to methods and compositions for inhibiting the expression of the DUX4 gene in skeletal muscle cells. In some aspects, the invention includes a CRISPR interference platform that directs an epigenetic regulator to the DUX4 locus. In some aspects, methods described by the disclosure are useful in modulating DUX4 expression for the treatment of facioscapulohumeral muscular dystrophy (FSHD).

Abstract: A hopper railroad car top hatch cover assembly including a hatch cover and a hatch cover securer configured to secure the hatch cover in a closed position engaging a coaming of the hopper railroad car.

Abstract: A hopper railroad car hatch cover including an elongated movable flexible hatch including an elongated flexible panel and an elongated magnetic coaming sealer connected to the bottom of the elongated movable flexible panel, wherein the elongated magnetic coaming sealer is configured, such that when the elongated movable hatch of the hatch cover is in the closed position on a coaming of a hopper railroad car, the elongated magnetic coaming sealer engages and creates a seal with the coaming.

Abstract: The disclosure relates to methods and compositions for regulating expression of DUX4. Specifically, the disclosure provides a recombinant gene editing complex comprising: a recombinant gene editing protein; and, a nucleic acid encoding a guide RNA (gRNA) that specifically hybridizes to a target nucleic acid sequence encoding a D4Z4 macrosatellite repeat region, wherein binding of the complex to the target nucleic acid sequence results in inhibition of DUX4 gene expression. In some aspects, methods described by the disclosure are useful for treating a disease associated with aberrant DUX4 expression (e.g., facioscapulohumeral muscular dystrophy, FSHD).

Abstract: A configurable railroad car including a plurality of multi-orientation coil/slab supports that are configured to be positioned on, oriented on, and securely attached to the floor of the railroad car in different sets of positions and orientations to enable the railroad car to transport different products such as coils, elongated slabs, a mix of coils and elongated slabs, or other products.

Abstract: The disclosure relates to methods and compositions for regulating expression of DUX4. In some aspects, methods described by the disclosure are useful for treating a disease associated with aberrant DUX4 expression (e.g., facioscapulohumeral muscular dystrophy).

Abstract: The disclosure relates to methods and compositions for regulating expression of DUX4. In some aspects, methods described by the disclosure are useful for treating a disease associated with aberrant DUX4 expression (e.g., facioscapulohumeral muscular dystrophy).

Abstract: Various embodiments provide a hopper railroad car top hatch cover assembly including a hatch cover and a hatch cover securer configured to secure the hatch cover in a closed position engaging a coaming of the hopper railroad car. Various embodiments provide a hopper railroad car having a top hatch cover assembly including a hatch cover and a hatch cover securer configured to secure the hatch cover in a closed position engaging a coaming of the hopper railroad car.

Abstract: The disclosure relates to methods and compositions for regulating expression of DUX4. In some aspects, methods described by the disclosure are useful for treating a disease associated with aberrant DUX4 expression (e.g., facioscapulohumeral muscular dystrophy).

Abstract: An auto-rack railroad car including a frame, a plurality of upright posts supported by the frame, a deck, and a plurality of deck connector assemblies connecting the deck to the upright posts, each deck connector assembly including at least one wedge.