Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.

Abstract: A regulating valve for a heat-transferring medium has a valve member controllable primarily by an electrical actuating member, but in the event of failure of the electrical actuating member, a thermostatic actuating member, operating independently of the electrical actuating member and of electric power, is provided in order to prevent damage to the system by driving the valve member when a predetermined temperature is exceeded.

Abstract: An improved unified cooling system for an internal combustion engine that generally includes a cylinder head and a cylinder block, in which the cylinder head and cylinder block are maintained at different operating temperatures despite using only a single coolant pump. The coolant pump pumps fresh coolant through the cylinder head and cylinder block. Coolant valves, which may be thermostatic valves or electrically actuable proportional valves, lie downstream of the cylinder head and cylinder block and individually control the amount of coolant flowing through the cylinder head and cylinder block so as to maintain a lower operating temperature for the cylinder head than for the cylinder block. Coolant is directed, in various configurations, through any or all of an oil cooler, a heat exchanger for a heater for a vehicle interior, and a coolant radiator, before being directed back to the coolant pump.

Abstract: An improved unified cooling system for an internal combustion engine that generally includes a cylinder head and a cylinder block, in which the cylinder head and cylinder block are maintained at different operating temperatures despite using only a single coolant pump. The coolant pump pumps fresh coolant through the cylinder head and cylinder block. Coolant valves, which may be thermostatic valves or electrically actuable proportional valves, lie downstream of the cylinder head and cylinder block and individually control the amount of coolant flowing through the cylinder head and cylinder block so as to maintain a lower operating temperature for the cylinder head than for the cylinder block. Coolant is directed, in various configurations, through any or all of an oil cooler, a heat exchanger for a heater for a vehicle interior, and a coolant radiator, before being directed back to the coolant pump.

Abstract: A regulating valve for a heat-transferring medium has a valve member controllable primarily by an electrical actuating member, but in the event of failure of the electrical actuating member, a thermostatic actuating member, operating independently of the electrical actuating member and of electric power, is provided in order to prevent damage to the system by driving the valve member when a predetermined temperature is exceeded.

Abstract: A thermostatic valve arranged in the coolant circuit of an internal-combustion engine, has a main valve which is acted upon by cold coolant flow on the one side, and by hot coolant flow on the other side. The valve is actuated as a function of the coolant temperature by way of an expansion material element equipped with an electric heating device. To minimize the coldside influence onto the expansion material element, a capsule which stores the expansion material, has an end which limits the expansion material lower at the level of the closed main valve or on the mixing chamber side. At this end, a ring-shaped or sleeve-shaped heating element is arranged coaxially adjacent the control pin, or, individual heating elements are distributed along the circumference of the control pin in an adjacent manner.

Abstract: To detect the capability to function of a thermostatic valve of a cooling system of an internal combustion engine of a vehicle, the actual temperature of the internal combustion engine, the ambient temperature and the actual temperature at the outlet of a coolant radiator are measured and evaluated in such a way that a malfunction of the thermostatic valve can be detected thereby.

Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.

Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.