Abstract: A high affinity, triplex-forming oligonucleotide and methods for use thereof wherein an oligonucleotide is used to form a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the binding of the oligonucleotide stimulates mutagenesis within or adjacent to the target sequence using cellular DNA synthesis or repair mechanisms thereby producing heritable changes in a human or animal. The mutation activates, inactivates or alters the activity and function of the target molecule. This mutation may be the result of a recombinagenic mechanism induced by the oligonucleotide.

Abstract: Methods for intracellularly generating single stranded DNA molecules that are active in mediating triplex-dependent or independent chromosomal events are provided herein. The method is based on the discovery that one can introduce viral vectors or plasmids into the cells, where they generate within the cells to be engineered the single stranded DNA molecules that bind to the target chromosomal DNA to form a triplex, which may induce a desired mutation, and/or be recombinagenic and induce a change to the chromosomal DNA by incorporation of a donor DNA molecule. The vectors or plasmid not only encode the TFO and optionally the donor DNA, but also a reverse transcriptase, and optionally, a restriction enzyme, which is present in the preferred embodiment as a fusion protein which reverse transcribes the RNA encoded by the vector or plasmid, then cleaves it at a restriction enzyme site to yield a single stranded DNA.

Abstract: A mutagenic, triplex-forming oligonucleotide and methods for use thereof wherein the oligonucleotide is chemically modified to incorporate a mutagen and forms a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the mutagen is brought into proximity with the target molecule and causes a mutation at a specific site therein. The mutation activates, inactivates or alters the activity and function of the target molecule.

Abstract: A high affinity, triplex-forming oligonucleotide and methods for use thereof wherein an oligonucleotide is used to form a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the binding of the oligonucleotide stimulates mutagenesis within or adjacent to the target sequence using cellular DNA synthesis or repair mechanisms. The mutation activates, inactivates or alters the activity and function of the target molecule.

Abstract: A high affinity, triplex-forming oligonucleotide and methods for use thereof wherein an oligonucleotide is used to form a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the binding of the oligonucleotide stimulates mutagenesis within or adjacent to the target sequence using cellular DNA synthesis or repair mechanisms. The mutation activates, inactivates or alters the activity and function of the target molecule.

Abstract: Methods, compositions and kits for effecting homologous recombination are described. In one aspect, the method utilizes two introduced DNAs: (1) a mutagen-linked single-stranded oligonucleotide capable of specifically binding to double-stranded DNA to form a triple-stranded helix, and (2) a donor DNA fragment capable of undergoing homologous recombination with DNA targeted by the oligonucleotide.