Abstract: The instant invention relates to solutions and methods that can be used to present the user of an analyser with a test result without resorting to human blood or plasma and thereby overcoming the disadvantages of using blood or plasma each time an analyser is to be used. In some embodiments, a solution for use with one or more blood coagulation sensor substrates to generate a signal relating to the clot time is provided, wherein the solution is non-biohazardous.