Abstract: A process for detecting cells involves applying a liquid, cell-containing sample to a porous support so that the cells enter the support pores and are retained by a cell-specific binding molecule on the pore surface and, optionally after the cells are detectably labeled, performing an optical read-out by high density imaging the entire porous support volume using an optical system having an optical axis that runs in the direction of sample flow.

Abstract: A process for detecting cells from a sample, comprising the following steps: (a) applying the sample or a fraction of the sample in liquid phase in a direction of flow to a porous, generally two-dimensional support with pores having on the surface thereof at least one binding molecule specific for the cells to be detected or for fragments of such cells; (b) allowing the cells to be detected or of fragments of such cells to enter from the sample or sample fraction into the pores of said porous, generally two-dimensional support, said pores having such a mean pore size that the cells or fragments to be detected are able to enter into the pores; (c) retaining the cells or fragments to be detected by at least one binding molecule specific for the cells or fragments to be detected; (d) performing an optical read-out method on said substantially two-dimensional support by an imaging method, optionally after the cells or cell fragments to be detected have been labeled with a labeling agent; wherein (e) the optica

Abstract: An in vitro process for the preparation of antibodies of the IgG type, comprising the steps of: i. challenging dendritic cells obtained from a donor with an antigen against which the antibodies to be prepared are directed; ii. challenging CD4+ T cells obtained from the same donor with fragments of the antigen presented by the dendritic cells from step L, with the proviso that the dendritic cells are ones essentially not secreting interferon-? (IFN-?) and interleukin 12 (IL-12), to generate antigen-specific CD4+ Th2 cells; iii. challenging with the antigen a B cell population obtained from the same donor and including a sufficient proportion of naive B cells; and iv. contacting the antigen-specific CD4+ Th2 cells according to ii. with at least a fraction of the B cell population from iii. to form an antigen-specific plasma cell; v. immortalizing said antigen-specific plasma cell; and vi. isolating the IgG antibodies formed by said antigen-specific plasma cell.

Abstract: The invention relates to a method for selectively detecting and/or quantifying superparamagnetic and/or ferromagnetic particles on analytes. The method is characterized in that a frequency component of magnetic fields (15, 18), which is generated due to the non-linearity of the magnetization characteristic curve of the particles, is measured at a mixing frequency. A device for selectively detecting and/or quantifying superparamagnetic and/or ferromagnetic particles on analytes comprises: a container (12) that contains particles, which are to be detected and/or quantified, on analytes; at least one oscillator (13, 16; 25) for generating frequencies of alternating magnetic fields (15, 18); at least one field generator (14, 17) for subjecting the analytes to alternating magnetic fields (15, 18); a magnetic field sensor (20) for measuring a response magnetic field (19) of the particles, and; at least one phase-sensitive detector (21, 23).

Abstract: The invention relates to a method for selectively detecting and/or quantifying superparamagnetic and/or ferromagnetic particles on analytes. The method is characterized in that a frequency component of magnetic fields (15, 18), which is generated due to the non-linearity of the magnetization characteristic curve of the particles, is measured at a mixing frequency. A device for selectively detecting and/or quantifying superparamagnetic and/or ferromagnetic particles on analytes comprises: a container (12) that contains particles, which are to be detected and/or quantified, on analytes; at least one oscillator (13, 16; 25) for generating frequencies of alternating magnetic fields (15, 18); at least one field generator (14, 17) for subjecting the analytes to alternating magnetic fields (15, 18); a magnetic field sensor (20) for measuring a response magnetic field (19) of the particles, and; at least one phase-sensitive detector (21, 23).

Abstract: The invention relates to a device in which at least two liquid or semi-liquid reagents can be stored separately. When used, the inventive device enables precise, chronologically defined, sequential reagent discharge in a reaction chamber by means of a linear movement without any prior mixing of said reagents.

Abstract: The invention relates to a device in which at least two liquid or semi-liquid reagents can be stored separately. When used, the inventive device enables precise, chronologically defined, sequential reagent discharge in a reaction chamber by means of a linear movement without any prior mixing of said reagents.

Abstract: The preparation of optically-active cyanohydrins from the corresponding oxo compounds is disclosed. The reaction of the oxo compounds with hydrocyanic acid is carried out in an organic solvent in the presence of (R)- or (S)-oxynitrilase (4.1.2.10) and (4.1.2.11), respectively, being solubilized in a lyotropic liquid crystal. Compounds which upon hydrolysis produce increased pH values are excluded as surface active agents. Preferably, surface active agents, the organic solvent and an aqueous buffer solution with a pH of 3 to 6, are mixed together to obtain a liquid crystal/organic solvent two-phase system. The liquid crystal is preferably fixed on a porous support, in particular a glass support. The reaction is carried out in a flow-through reactor which contains the liquid crystal in the abovementioned form or in thin layers adjacent to narrow flow channels, the borders of which are liquid permeable and through which the substrate-containing solvent is passed.

Abstract: A biocatalytic method for reacting organic, especially poorly water-soluble substances. Enzymes, prokariotic and eukariotic cells or combinations of enzymes and cells are immobilized in lyotropic liquid crystals (mesophases), preferably with inverse phase structure. The supply of substrate and the removal of product are performed via one or more solvent phases. Organic solvents function as solvent phases. In addition, an aqueous solvent phase can also be present. In order to produce these two and three-phase systems, three-component systems consisting of water, organic solvent and surfactant are used.