Abstract: The invention relates to a method for the specific detection of a microorganism or a group of microorganisms via in situ hybridisation by means of flow cytometry.

Abstract: The invention relates to a method for the specific detection of a microorganism or a group of microorganisms via in situ hybridisation by means of flow cytometry.

Abstract: The present invention describes an in situ hybridization arrangement for the specific detection of microorganisms in a sample. Such arrangement has a container with at least one opening, a support for the hybridization solution, a slide, and a fastening means for the slide. The invention further describes a method for specific detection of microorganisms in a sample by in situ hybridization. The method of the present invention comprises of the steps of: fixing the microorganisms contained in the sample; incubating the fixed cells with detectable nucleic acid probe molecules; removing or washing-off the non-hybridized nucleic acid probe molecules, and detecting the cells hybridized with the nucleic acid probe molecules. Such method is carried out using the in situ hybridization arrangement of the invention.