Abstract: The present invention relates to a method for preventing or treating a cancer disease by targeting the epigenetic factor Chromodomain on Y-like 2 (CDYL2). The inventors found that CDYL2 is commonly over-expressed in cancer and high CDYL2 levels correlate with poor prognosis in a number of cancer types even in drug resistant cancer. CDYL2 upregulation in a breast cancer cell line induced migration, invasion, stem-like phenotypes, as well as an epithelial-to-mesenchymal transition (EMT). Due to the importance of EMT and stemness in therapeutic resistance and relapse in cancer, the inventors propose that CDYL2 inhibition will also be beneficial to the treatment of such cancers. Furthermore RNAi inhibition of CDYL2 diminished these same EMT-associated processes in the mesenchymal-like breast cancer cell line.

Abstract: In some embodiments the disclosure is directed to a closed-loop piston engine system using a recirculating carbon dioxide (CO2) system with supercritical carbon dioxide (scCO2) as a working fluid. The closed-loop piston engine system may include a scCO2 injector; a superheating nozzle region; a first valve; a second valve; a piston moving in the cylinder and coupled with a crankshaft, the piston being driven toward a centerline of the crankshaft during a power stroke using a connecting rod and causing the crankshaft to rotate thereby causing one power stroke per piston per crankshaft rotation and thereby producing two power strokes for every single power stroke that a similar engine would produce if run as a hydrocarbon fuel powered internal combustion engine. The recirculating CO2 system recirculates the used carbon dioxide and there are no carbon dioxide emissions from the system.

Abstract: In some embodiments the disclosure is directed to a closed-loop piston engine system using a recirculating carbon dioxide (CO2) system with supercritical carbon dioxide (scCO2) as a working fluid. The closed-loop piston engine system may include a scCO2 injector; a superheating nozzle region; a first valve; a second valve; a piston moving in the cylinder and coupled with a crankshaft, the piston being driven toward a centerline of the crankshaft during a power stroke using a connecting rod and causing the crankshaft to rotate thereby causing one power stroke per piston per crankshaft rotation and thereby producing two power strokes for every single power stroke that a similar engine would produce if run as a hydrocarbon fuel powered internal combustion engine. The recirculating CO2 system recirculates the used carbon dioxide and there are no carbon dioxide emissions from the system.

Abstract: In some embodiments the disclosure is directed to a closed-loop piston engine system using a recirculating carbon dioxide (CO2) system with supercritical carbon dioxide (scCO2) as a working fluid. The closed-loop piston engine system may include a scCO2 injector; a superheating nozzle region; a first valve; a second valve; a piston moving in the cylinder and coupled with a crankshaft, the piston being driven toward a centerline of the crankshaft during a power stroke using a connecting rod and causing the crankshaft to rotate thereby causing one power stroke per crankshaft rotation and thereby producing two power strokes for every single power stroke that a similar engine would produce if run as a hydrocarbon fuel powered internal combustion engine. The recirculating CO2 system recirculates the used carbon dioxide and there are no carbon dioxide emissions from the system.

Abstract: In some embodiments the disclosure is directed to a closed-loop piston engine system using a recirculating carbon dioxide (CO2) system with supercritical carbon dioxide (scCO2) as a working fluid. The closed-loop piston engine system may include a scCO2 injector; a superheating nozzle region; a first valve; a second valve; a piston moving in the cylinder and coupled with a crankshaft, the piston being driven toward a centerline of the crankshaft during a power stroke using a connecting rod and causing the crankshaft to rotate thereby causing one power stroke per crankshaft rotation and thereby producing two power strokes for every single power stroke that a similar engine would produce if run as a hydrocarbon fuel powered internal combustion engine. The recirculating CO2 system recirculates the used carbon dioxide and there are no carbon dioxide emissions from the system.