Abstract: The present invention relates to a method for obtaining crystalline 2?-fucosyllactose from a 2?-FL raw material, which contains 2?-FL as a main constituent and at least 0.

Abstract: Increasing space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility in the production of fine chemicals The inventors of the current invention have found a surprising positive effect of increased cAMP levels and/or manipulating the PTS system on the space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility of fine chemical production of a host organism. This was achieved by de-regulating adenylate cyclase cyaa by deleting the C-terminal regulatory region leading to increased cAMP levels or deleting the Crr protein activity (carbohydrate repression resistance) which regulates the carbohydrate utilization system. Both lead to increased 2-fucosyllactoe and 6-sialyllactose production (human milk oligosaccharides) and increase carbohydrate usage.

Abstract: The present invention relates to a method of storing a biocatalyst, which biocatalyst is capable of converting acrylonitrile to acrylamide, comprising the steps: (a) providing an aqueous suspension comprising the biocatalyst, which is capable of converting acrylonitrile to acrylamide, which aqueous suspension is an aqueous fermentation broth; (b) sequentially in either order or simultaneously (b) concentrating the aqueous suspension comprising the biocatalyst to a concentration of at least 3% (w/w); and (b2) reducing the temperature of the aqueous suspension comprising the biocatalyst to a temperature of below 8° C., thereby forming a concentrated aqueous suspension; and (c) maintaining the concentrated aqueous suspension of step (b) at a temperature of below 8° C. The invention also relates to a biocatalyst composition obtainable by this method and also the use of the biocatalyst composition in a process of preparing (meth-) acrylamide aqueous solution from (meth-) acrylonitrile.

Abstract: The present invention relates to a method for separating biomass from a solution comprising biomass and at least one oligosaccharide.comprising providing the solution comprising biomass and oligosaccharides.lowering the pH value of the solution below 7 by adding at least one acid to the solution comprising biomass and the at least one oligosaccharide. adding an adsorbing agent to the solution comprising biomass and oligosaccharides. and carrying out first membrane filtration so as to separate the biomass from the solution comprising the at least one oligosaccharide.

Abstract: The present invention relates to a method for obtaining crystalline 2?-fucosyllactose from a 2?-FL raw material, which contains 2?-FL as a main constituent and at least 0.

Abstract: The present invention relates to a process for preparing ammonium (meth-) acrylate, aqueous ammonium (meth-) acrylate solutions obtainable by such process, and (meth-) acrylic acid homopolymers or copolymers obtainable by polymerizing such ammonium (meth-) acrylate. The invention furthermore relates to a modular, relocatable bioconversion unit for manufacturing aqueous ammonium (meth-) acrylate solutions.

Abstract: The present invention relates to means and methods for producing an amide compound from a nitrile compound with less acrylic acid as by-product using a Nitrile hydratase (NHase) and Amidase producing microorganism as biocatalyst. Also provided is an aqueous amide compound obtained by the methods of the invention as well as a composition comprising acrylamide or polyacrylamide as well as a dried microorganism exhibiting a NHase/Amidase activity ratio of at least 400 when being brought into contact with a nitrile compound to convert said nitrile compound into an amide compound.

Abstract: The present invention relates to methods for preparing an aqueous acrylamide solution having a low acrylic acid concentration. In addition, the present invention relates to methods for reducing the acrylic acid concentration of an aqueous acrylamide solution. The methods involve a bioconversion of acrylonitrile to acrylamide in the presence of a biocatalyst, wherein during the bioconversion the content of acrylonitrile is maintained at 0.3 w/w % or more referred to the total weight of the composition in the reactor. Also provided is an aqueous acrylamide solution which is obtained by the methods of the present invention. Furthermore, the present invention is related to an acrylamide homopolymer or copolymer obtained by polymerizing the acrylamide of the aqueous solution.

Abstract: The invention pertains to processes to produce dry biomass of pyripyropene producer organisms, processes to obtain pyripyropenes from such dry biomass, as well as to processes to produce compounds of Formula III and/or Formula IV and/or Formula V from the pyripyropenes obtained from the dry biomass. The invention does further pertain to the dry biomass itself, as well as processes using said dry biomass to obtain pyripyropenes for the production of compounds of Formula III and/or Formula IV and/or Formula V, including processes using said dry biomass to obtain pyripyropenes or compounds of Formula III and/or Formula IV and/or Formula V in order to produce pest control compositions, in particular insecticides, comprising such compounds.

Abstract: The present invention relates to methods for preparing an aqueous acrylamide solution having a low acrylic acid concentration. In addition, the present invention relates to methods for reducing the acrylic acid concentration of an aqueous acrylamide solution. The methods involve a bioconversion of acrylonitrile to acrylamide in the presence of a biocatalyst, wherein during the bioconversion the content of acrylonitrile is maintained at 0.3 w/w % or more referred to the total weight of the composition in the reactor. Also provided is an aqueous acrylamide solution which is obtained by the methods of the present invention. Furthermore, the present invention is related to an acrylamide homopolymer or copolymer obtained by polymerizing the acrylamide of the aqueous solution.

Abstract: The present invention relates to methods for cultivating a nitrile hydratase producing microorganism, compositions for cultivating a nitrile hydratase producing microorganism, and use of compositions comprising a saccharide and an organic acid for cultivating a nitrile hydratase producing microorganism. The composition provided in and to be employed in context with the present invention is particularly suitable for inducing both, growth and nitrile hydratase production of corresponding microorganisms.

Abstract: The present invention relates to means and methods for producing an amide compound from a nitrile compound with less acrylic acid as by-product using a Nitrile hydratase (NHase) and Amidase producing microorganism as biocatalyst. Also provided is an aqueous amide compound obtained by the methods of the invention as well as a composition comprising acrylamide or polyacrylamide as well as a dried microorganism exhibiting a NHase/Amidase activity ratio of at least 400 when being brought into contact with a nitrile compound to convert said nitrile compound into an amide compound.

Abstract: The invention pertains to processes to produce dry biomass of pyripyropene producer organisms, processes to obtain pyripyropenes from such dry biomass, as well as to processes to produce compounds of Formula III and/or Formula IV and/or Formula V from the pyripyropenes obtained from the dry biomass. The invention does further pertain to the dry biomass itself, as well as processes using said dry biomass to obtain pyripyropenes for the production of compounds of Formula III and/or Formula IV and/or Formula V, including processes using said dry biomass to obtain pyripyropenes or compounds of Formula III and/or Formula IV and/or Formula V in order to produce pest control compositions, in particular insecticides, comprising such compounds.