Abstract: The present disclosure provides a system for cell engineering target cells in a mixture that includes the target cells and non-target cells, the system including: i) a cell monitoring component for tracking and classifying individual cells in the mixture, wherein classifying a cell comprises determining whether the cell is a target cell; ii) an engineering component for selectively modifying individual cells at one or more engineering sites, wherein the target cells and the non-target cells are randomly distributed in the engineering component; and iii) a control component for controlling the engineering component so as to selectively modify only target cells tracked to be at one of the engineering sites.

Abstract: In a one aspect, a cell engineering module is provided for cell engineering target cells in a mixture comprising the target cells and non-target cells. The cell engineering module includes: i) a cell monitoring zone for tracking and classifying individual cells in the mixture, wherein classifying a cell includes determining whether the cell is a target cell; and ii) an engineering component in the cell monitoring zone to selectively modify individual cells at one or more engineering sites, wherein the target cells and the non-target cells may be randomly distributed in the engineering component; wherein the engineering component is operable to selectively modify only target cells that have been tracked to be at one of the engineering sites.

Abstract: A system for label-free identification and/or classification and/or selection of at least one cell is provided. The system comprises a microfluidic environment configured to be traversable by the at least one cell, an imaging sensor configured to gather imaging information with respect to at least a part of the microfluidic environment, and a processing unit connected to the imaging sensor. In this context, the microfluidic environment comprises at least one sub-environment configured to interact with the at least one cell. Furthermore, the processing unit is configured to detect at least one sub-environment interaction event based on persistence of the at least one cell imaged by the imaging sensor in the temporal domain. In addition to this, the processing unit is configured to identify and/or classify and/or select the at least one cell based on the at least one sub-environment interaction event.

Abstract: The present invention provides a micro-reactor (1) adapted to host chemical reactions having at least one microfluidic layer, said micro-reactor (1) comprising a fluid inlet (2) and a fluid outlet (3); a plurality of micro-reaction chambers (10) arranged in rows (7) and columns (6), each micro-reaction chamber comprising a chamber inlet (10a) and a chamber outlet (10b); a plurality of supply channels (4) for supplying fluid to from said fluid inlet (2) to said micro-reaction chambers (10) and further arranged for draining said micro-reaction chambers (10) to said fluid outlet (3), said supply channels (10) extending in a first direction (D1) along the columns (6) of micro-reaction chambers (10) and arranged such that there is one supply channel (4) between adjacent columns (6).

Abstract: A semiconductor cell culture device for three-dimensional cell culture comprises: a semiconductor material layer in which a cell culture portion of semiconductor material is defined, wherein the cell culture portion defines an area within the semiconductor material layer surrounded by semiconductor material, wherein the cell culture portion comprises a mesh structure having island structures being interconnected by bridge structures and defining through-pores between the island structures allowing for selective transport of cell constructs, cellular components, proteins or other large molecules through the semiconductor material layer and on opposite sides of the cell culture portion in the semiconductor material layer, and a supporting structure connected to the cell culture portion.

Abstract: A device for sorting biological cells comprises a flow channel configured to pass a flow of liquid carrying the biological cells. The flow channel comprises at least one zone associated with a cell category. Each zone of the flow channel comprises at least one surface coated with molecules having an affinity to the cell category associated with the zone, the at least one surface of the zone being configured to modify, by the molecules of the surface, a movement of a cell belonging to the associated cell category of the zone as the cell passes the zone. The device comprises a detector configured to detect a cell exhibiting at least one modified movement in one zone of the flow channel, and to transmit a trigger signal based on the detection of the cell. The device further comprises an actuator configured to divert the detected cell based on the trigger signal.

Abstract: A method for monitoring a monitor material of a process, such as a manufacturing process, comprises: detecting a suspicious particle in a sample of the monitor material using an imaging system configured to image the sample using illumination light from a light source and to detect an interference pattern based on object light having interacted with the sample and reference light of the illumination light; selectively diverting the suspicious particle rom a flow of the sample to an analysis flow; activating a nucleic acid test (NAT) device, wherein said activating is triggered based on detecting of the suspicious particle in the sample; receiving by the NAT device the analysis flow comprising the suspicious particle; and subjecting the suspicious particle to a NAT analysis. Also, an apparatus for monitoring a monitor material is provided.

Abstract: A collecting device (200) for collection of particles and presentation of collected particles for analysis comprises: a first layer (202) and a second layer (220) spaced apart for defining a particle collection chamber (240); wherein the first layer (202) is configured to receive a flow of air (104) carrying airborne particles, wherein the first layer (202) comprises a plurality of inlet nozzles (210) configured to extend through the first layer (202) for transporting the flow of air (104) therethrough; wherein the inlet nozzles (210) are configured to face a first surface (222) of the second layer (220) for capturing airborne particles in the flow of air (104) entering the particle collection chamber (240) by impaction of airborne particles; wherein the collecting device (200) is configured to provide optical access for performing a measurement, based on light, of airborne particles collected in the particle collection chamber (240).

Abstract: A collecting device (200) for collecting airborne particles comprises: a first (202) and second layer (220) spaced apart for forming a particle collection chamber (240) therebetween, wherein inlets (210) extend through the first layer (202) for transporting a flow of air into the particle collection chamber (240); wherein ends (214) of the inlets (210) face a first surface (222) of the second layer (220) for capturing airborne particles by impaction; wherein outlets (230) extend through the second layer (220) for transporting the flow of air out of the particle collection chamber (240); wherein the inlets (210) and outlets (230) are staggered such that the center axes of the inlets (210) and outlets (230) are displaced from each other; wherein the flow of air experiences a pressure drop lower than 3 kPa at a flow rate of 0.5 liters per second, when the flow of air passes the collecting device (200).

Abstract: The present disclosure relates to a fluid analyzing device that includes a sensing device for analyzing a fluid sample. The sensing device includes a microchip configured for sensing the fluid sample, and a closed micro-fluidic component for propagating the fluid sample to the microchip. The fluid sample can be provided to the micro-fluidic component via an inlet of the fluid analyzing device. And a vacuum compartment, which is air-tight connected to the sensing device, can create in the micro-fluidic component a suction force suitable for propagating the fluid sample through the micro-fluidic component.

Abstract: There is provided a connecting interface for fluidically interconnecting microfluidic channels. The connecting interface comprises one or more substrates which collectively define a first microfluidic channel which includes a connecting region for fluidically connecting the first microfluidic channel to a second microfluidic channel. The connecting interface further comprises at least one slit in an outer surface of one of the one or more substrates, wherein the at least one slit provides a fluid passage from the outer surface to the connecting region of the first microfluidic channel, and the at least one slit has at least one dimension extending beyond the connecting region along a direction parallel to the outer surface.

Abstract: A microfluidic device comprising a plurality of microreactors is provided. Each microreactor includes at least a first inlet and a second inlet for supplying a first fluid and a second fluid, respectively, to said microreactor and at least one waste channel for draining fluid from said microreactor. The device further comprises a shared first microfluidic supply system for supplying a first fluid to the first inlets of the plurality of microreactors, a shared second microfluidic supply system for supplying a second fluid to the second inlets of the plurality of microreactors. At least one of said inlets to each microreactor comprises at least one valve-less fluidic resistance element having a fluidic resistance that is substantially larger than the fluidic resistance of the corresponding shared microfluidic supply system. A chemical reaction sequencer apparatus including the microfluidic device and a method for supplying reagents to a plurality of microreactors are also provided.

Abstract: The present invention provides a micro-reactor (1) adapted to host chemical reactions having at least one microfluidic layer, said micro-reactor (1) comprising a fluid inlet (2) and a fluid outlet (3); a plurality of micro-reaction chambers (10) arranged in rows (7) and columns (6), each micro-reaction chamber comprising a chamber inlet (10a) and a chamber outlet (10b); a plurality of supply channels (4) for supplying fluid to from said fluid inlet (2) to said micro-reaction chambers (10) and further arranged for draining said micro-reaction chambers (10) to said fluid outlet (3), said supply channels (10) extending in a first direction (D1) along the columns (6) of micro-reaction chambers (10) and arranged such that there is one supply channel (4) between adjacent columns (6).

Abstract: The present disclosure relates to a spectral sensor for detection of individual light-emitting particles. The sensor is comprising an array of photo-sensitive detectors for detecting light emitted by said individual light-emitting particles and a filter array comprising a plurality of different band-stop filters. The filter array is configured to transmit wavelengths in a detectable wavelength region to the array of photo-sensitive detectors, and wherein each band-stop filter is associated with one or more particular photo-sensitive detectors, and the plurality of different band-stop filters are configured to reflect different wavelength intervals within said detectable wavelength region so that each photo-sensitive detector of the array is configured to detect the wavelengths of the detectable wavelength region other than the reflected wavelength interval of the band-stop filter being associated with the photo-sensitive detector.

Abstract: The present disclosure relates to devices and methods for analyzing a fluid sample. An example device comprises a fluidic substrate comprising a micro-fluidic component embedded therein, for propagating a fluid sample; a needle or inlet for providing the fluid sample which is fluidically connected to the micro-fluidic component; a lid attached to the fluidic substrate thereby at least partly covering the fluidic substrate and at least partly closing the micro-fluidic component; wherein the fluidic substrate is a glass fluidic substrate and wherein the lid is a microchip. The present disclosure also relates to a method for fabricating a fluid analysis device. The method comprises providing a fluidic substrate; providing a lid; attaching the lid to the fluidic substrate to close the fluidic substrate at least partly.

Abstract: The present disclosure relates to devices and methods for non-invasive measuring of analytes. At least one embodiment relates to a wearable system for non-invasive measuring of a concentration of an analyte in skin tissue. The wearable system includes an integrated circuit that includes a first optical unit. The first optical unit includes a Raman spectrometer. The first optical unit also includes an OCT spectrometer and an interferometer optically coupled to the OCT spectrometer or an infrared (IR) spectrometer. The first optical unit additionally includes a light coupler. The wearable system further includes a first light source for performing Raman spectroscopy. The wearable system additionally includes a second light source for performing OCT spectroscopy or IR spectroscopy. Still further, the wearable system includes read-out electronics to determine an optical model of the skin tissue based on the spectroscopic data and to determine the concentration of the analyte.

Abstract: A semiconductor cell culture device for three-dimensional cell culture comprises: a semiconductor material layer in which a cell culture portion of semiconductor material is defined, wherein the cell culture portion defines an area within the semiconductor material layer surrounded by semiconductor material, wherein the cell culture portion comprises a mesh structure having island structures being interconnected by bridge structures and defining through-pores between the island structures allowing for selective transport of cell constructs, cellular components, proteins or other large molecules through the semiconductor material layer and on opposite sides of the cell culture portion in the semiconductor material layer, and a supporting structure connected to the cell culture portion.

Abstract: A sensor device for quantifying luminescent targets comprises a light source, a detector, a modulator, and a processor. The light source is adapted for exciting the luminescent target. The detector is adapted for detecting the luminescence of the luminescent target resulting in a measured signal which comprises a desired signal originating from the luminescent target and a background signal. The modulator is adapted for modulating a physical parameter resulting in a modulation of the desired signal which is different from the modulation of the background signal. The processor is configured to correlate the modulation of the physical parameter with the modulation of the desired signal and/or the modulation of the background signal.

Abstract: Sensor devices for quantifying luminescent targets are described herein. An example device comprises a light source for exciting the targets, thus generating luminescence signals and a detector for detecting these signals, resulting in a detected signal which comprises a desired signal originating from the targets and a background signal. It moreover comprises a bleaching device for bleaching of at least part of the sources generating the background signal and a processor configured to trigger the bleaching device to start bleaching, and to trigger the light source for exciting the remaining luminescent targets which are not bleached, and to trigger the detector for detecting the luminescence signal of the remaining luminescent targets, so as to generate a measurement signal representative for the quantification of the luminescent targets.

Abstract: The present disclosure relates to a device for analyzing a fluid sample. In one aspect, the device includes a fluidic substrate that comprises a micro-fluidic component embedded in the fluidic substrate configured to propagate a fluid sample via capillary force through the device and a means for providing a fluid sample connected to the micro-fluidic component. The device also includes a lid attached to the fluidic substrate at least partly covering the fluidic substrate and at least partly closing the micro-fluidic component. The fluidic substrate may be a silicon fluidic substrate and the lid may be a CMOS chip. In another aspect, embodiments of the present disclosure relate to a method for fabricating such a device, and the method may include providing a fluidic substrate, providing a lid, and attaching, through a CMOS compatible bonding process, the fluidic substrate to the lid to close the fluidic substrate at least partly.