Abstract: The present description is related to the field of protein production. It introduces novel host cells with low protease activity, a novel protease regulator, its use in expression systems and protein production, and a method of producing host cells for protein production.

Abstract: The present description is related to the field of protein production. It introduces novel host cells with low protease activity, a novel protease regulator, its use in expression systems and protein production, and a method of producing host cells for protein production.

Abstract: The present description is related to the field of protein production.

Abstract: The invention relates to the use of fusion proteins between at least two plant cell-wall degrading enzymes, the enzymes being such that they do not contain a C-terminal carbohydrate-binding-molecule (CBM), and optionally a CBM, the enzymes and CBM being recombinant proteins corresponding to native proteins in fungi, or mutated forms thereof, for carrying out processes of plant cell-wall degradation in the frame of the preparation, from plants or vegetal by-products, of compounds of interest located in plant cell-wall, or in the frame of the bleaching of pulp and paper.

Abstract: The invention relates to the use of fusion proteins between at least two plant cell-wall degrading enzymes, the enzymes being such that they do not contain a C-terminal carbohydrate-binding-molecule (CBM), and optionally a CBM, the enzymes and CBM being recombinant proteins corresponding to native proteins in fungi, or mutated forms thereof, for carrying out processes of plant cell-wall degradation in the frame of the preparation, from plants or vegetal by-products, of compounds of interest located in plant cell-wall, or in the frame of the bleaching of pulp and paper.

Abstract: The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glyclosylation and protein folding. The invention provides for the use of fungi with altered morphology, which permits high-throughput screening and directed molecular evolution of expressed proteins. The same transformed fungi may be used to produce larger quantities of protein for isolation, characterization, and application testing, and may be suitable for commercial production of the protein as well.