Abstract: The application discloses adipose tissue-derived stem cells (ADSC) and related compositions and methods. ADSCs are useful for (i) production of insulin producing cells, (ii) treatment of diabetes, (iii) endothelial cell reconstitution, (iv) treatment of overactive bladder and urge incontinence, (v) prevention and treatment of bladder voiding dysfunction; (vi) treatment of neurogenic impotence such as that resulting from diabetes or after prostate cancer therapy; (vii) treatment of vasculogenic impotence, such as that resulting from hypertension, dyslipidemia, atherosclerosis and diabetes; (viii) the promotion of wound healing; (ix) reduction of skin wrinkling; and (x) hair growth.

Abstract: A system and method delivers and collects a wash medium into a lumen of the urinary tract system. Isolated saline delivery washes cells from a urethra that are collected for cancer diagnosis. BCG is delivered for local chemotherapy. The isolated delivery and collection is from a region bound by a distal occlusion and proximal occlusion. Inflatable balloons provide the occlusion and or a penis clamp may be used. The two balloons may telescope relative to each other. A balloon inflation lumen may collapse a valve membrane to occlude distal flow of infused fluid to only proximal of the balloon.