Abstract: A method and system for calibrating color filters employed in polychromatic imaging of a subject includes a scanning mirror (28), telescope (30), filters (104), and a detector array (60) employed for both imaging and calibration processes. A bundle (44) of optical fibers is employed for producing a slit-shaped beam of solar rays which are collimated and applied to a diffraction grating plate (54) or prism (72) to produce a set of dispersed solar rays. The dispersion is based on color. In one position of the scanning mirror, rays from a subject (12) to provide an image are directed through the telescope and scanned across the filters (104) and detectors (102). In another position of the scanning mirror, the set of dispersed solar rays is scanned past the filters and the detectors. Imaging data outputted by the detectors is collected for producing an image (112) of the subject. Data of the dispersed rays is collected for calibrating the color filters.