Abstract: The present invention relates to a method for the determination of the presence of PV-neutralizing antibodies in a sample, comprising a) contacting said sample with infectious PV particles comprising a reporter gene, wherein the gene product of said reporter gene is secreted into the growth medium, b) contacting the PV particles from a) with host cells, and c) determining PV-neutralizing antibodies based on the amount of gene product from said reporter gene, wherein, preferably, a lower amount of said gene product as compared to a reference amount is indicative of the presence of PV-neutralizing antibodies. It further relates to a host cell strongly adhering to multi-cluster plates for use in a method for diagnosing anti-PV immunity comprising the method of the present invention.

Abstract: The present invention relates to a generic capture ELISA for the detection and measurement of antibodies in biological fluids such as serum. This newly developed enzyme-linked immunosorbent assay (ELISA) system uses a first binding partner of a binding pair, preferably glutathione, crosslinked to casein as capture protein to bind recombinant protein antigens fused to a second binding partner of said binding pair, preferably N-terminal glutathione S-transferase (GST). The method not only allows the specific and efficient detection of antibodies in biological samples but, in addition, simple and efficient immobilization and one-step purificaton of overexpressed recombinant antigens even from crude lysates on ELISA plates coated with the first binding partner/casein. Several antigens can be tested in parallel under the same conditions without the need to biochemically purify or renature the proteins.

Abstract: A computerized voting information system that encompasses one or more voting stations, at least one tabulation center and certification center, and a plurality of voters so as to automatically verify, manipulate, interchange, and manage all data and information that is needed by the voting stations to determine if a particular voting card is authentic and a voter is entitled to cast his/her vote, by tabulation centers to perform the tabulation of the casted votes, by certification centers to guarantee the authenticity of the voting cards and the legitimacy of the card holders, and by voters to prove their identity and voting eligibilities.