Abstract: The invention relates to array-based methods for identification of neoepitope reactive T cells and to compositions produced using such methods. Aspects of the invention relate to rapid and reliable methods to identify neoepitope reactive T cells.

Abstract: A pharmaceutical compositions and methods for immunotherapy are provided. The pharmaceutical composition includes a genetically-engineered bacterium expressing a human disease-related antigen(s), preferably two or more patient-specific tumor antigens as a polytope. The bacterium has genetically engineered lipopolysaccharide or a patient's own endosymbiotic bacterium so that the bacterium expresses endotoxin at a low level, which is insufficient to induce a CD-14 mediated sepsis. The genetically-engineered bacterium can be administered to the patient, either systemically or locally, to induce tumor-specific immune response.

Abstract: Two-component vaccine formulations and methods are contemplated where the vaccine has an adjuvant component and a therapeutic component. The therapeutic component comprises preferably a recombinant therapeutic virus encoding a therapeutic antigen while the adjuvant component comprises a non-host cell or immune stimulating portion thereof. Notably, use of the adjuvant component will result in significant uptake of the therapeutic component into immune competent cells, even in the absence of receptors for entry of the therapeutic component. In addition, such adjuvant also stimulates expression of the therapeutic antigen.

Abstract: Methods and devices are provided for focusing and/or sorting activated T cells. The device comprises a microfluidic channel comprising a plurality of electrodes arranged to provide dielectrophoretic (DEP) forces that are perpendicular to forces from hydrodynamic flows along the channel. The device may be configured to apply voltages to a plurality of electrodes in a first upper region of the microfluidic channel to focus the cells into a single flow, and to apply different voltages to a plurality of electrodes in a second downstream region of the microfluidic channel to sort cells based on size. The output of the microfluidic channel may diverge into multiple channels, wherein cells of various sorted sizes are directed into the appropriate output channel.