Abstract: The present disclosure describes methods and systems for use in the production of adeno-associated virus (AAV) particles, including recombinant adeno-associated virus (rAAV) particles. In certain embodiments, the production process and system use Spodoptera frugiperda insect cells (such as Sf9 or Sf21) as viral production cells (VPCs).

Abstract: The present disclosure describes methods and systems for use in the production of adeno-associated virus (AAV) particles, including recombinant adeno-associated virus (rAAV) particles. The production process and system use Baculoviral Expression Vectors (BEVs) and/or Baculoviral Infected Insect Cells (BIICs) in the production of AAV particles (e.g., rAAVs) which allow for the controlled expression of AAV structural (e.g., capsid) proteins, such as VP1, VP2, and VPS and the controlled expression of AAV nonstructural (e.g., replication) proteins, such as Rep78 and Rep52.

Abstract: The present disclosure describes methods and systems for use in the production of adeno-associated virus (AAV) particles, compositions, and formulations, including recombinant adeno-associated viruses (rAAV). The present disclosure presents cell culture mediums for use in producing adeno-associated viruses (AAV), such as AAV which comprise a polynucleotide encoding a payload. In certain embodiments, the cell culture medium comprises a hydrolysate mixture, L-glutamine, poloxamer 188 (e.g., 10% pluronic F-68), a lipid emulsion, and a cholesterol mixture. In certain embodiments, the production process and system use Spodoptera fmgiperda insect cells (such as Sf9 or Sf21) as viral production cells (VPCs). In certain embodiments, the production process and system use Baculo viral Expression Vectors (BEVs) and/or Baculoviral Infected Insect Cells (BIICs) in the production of rAAVs.

Abstract: The present disclosure describes methods and systems for use in the production of adeno-associated virus (AAV) particles, including recombinant adeno-associated virus (rAAV) particles. In certain embodiments, the production process and system use Spodoptera frugiperda insect cells (such as Sf9 or Sf21) as viral production cells. In certain embodiments, the production process and system use Baculoviral Expression Vectors (BEVs) in the production of AAV particles. In certain embodiments, the production process and system allow for the controlled expression of AAV capsid proteins, such as VP1, VP2 and VP3.

Abstract: The present disclosure describes methods and systems for use in the production of adeno-associated virus (AAV) particles, compositions and formulations, including recombinant adeno-associated viruses (rAAV). The present disclosure presents cell culture mediums for use in producing adeno-associated viruses (AAV), such as AAV which comprise a polynucleotide encoding a payload. In certain embodiments, the cell culture medium comprises a hydrolysate mixture, L-glutamine, poloxamer 188 (e.g. 10% pluronic F-68), a lipid emulsion, and a cholesterol mixture. In certain embodiments, the production process and system use Spodoptera frugiperda insect cells (such as Sf9 or Sf21) as viral production cells (VPCs). In certain embodiments, the production process and system use Baculoviral Expression Vectors (BEVs) and/or Baculoviral Infected Insect Cells (BIICs) in the production of rAAVs.

Abstract: The present disclosure presents methods for producing baculovirus infected insect cells (BIICs). The present disclosure describes methods and systems for use in the production of adeno-associated virus (AAV) particles, compositions and formulations, including recombinant adeno-associated viruses (rAAV). In certain embodiments, the production process and system use Baculoviral Expression Vectors (BEVs) and/or Baculoviral Infected Insect Cells (BIICs) in the production of rAAVs. In certain embodiments, the present disclosure presents methods and systems for designing, producing, clarifying, purifying, formulating, filtering and processing rAAVs and rAAV formulations. In certain embodiments, the production process and system use Spodoptera frugiperda insect cells (such as Sf9 or Sf21) as viral production cells (VPCs).

Abstract: An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

Abstract: This disclosure includes devices, systems, and methods for identifying and tracking products to be obtained and/or tasks to be performed for a structure (e.g., for repair and/or remodeling of the structure). For example, some embodiments of the present portable computing devices comprise: a processor; an input device; a display and memory storing instructions executable by the processor to: present via the display an input interface having a plurality of input portions each associated with a different portion of a structure; receive via the input device user selections of products that are available form a supplier, at least some of the selected products each associated with a different portion of the structure; and generate an output of information associated with the selected products, the output including for each of the selected products a unique identifier corresponding to a product record of the supplier.

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

Abstract: An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.

Abstract: This disclosure includes devices, systems, and methods for identifying and tracking products to be obtained and/or tasks to be performed for a structure (e.g., for repair and/or remodeling of the structure). For example, some embodiments of the present portable computing devices comprise: a processor; an input device; a display and memory storing instructions executable by the processor to: present via the display an input interface having a plurality of input portions each associated with a different portion of a structure; receive via the input device user selections of products that are available form a supplier, at least some of the selected products each associated with a different portion of the structure; and generate an output of information associated with the selected products, the output including for each of the selected products a unique identifier corresponding to a product record of the supplier.

Abstract: An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.

Abstract: Methods and apparatus are provided which may be employed to perform speech recognition processing on a grid computing system. In some embodiments, the grid computing system includes a server system which receives processing tasks from one or more client applications, divides the processing tasks into units of work, and assigns the units of work to one or more of the nodes. Dividing a processing task into units of work may involve dividing audio input data into segments defined by natural speech boundaries. A mathematical representation may be created for each segment prior to its distribution on the grid to minimize network traffic. A node in the system may perform heterogeneous units of work concurrently, such as by isolating the execution of each unit of work in an application domain.

Abstract: Methods and apparatus are provided which may be employed to perform speech recognition processing on a grid computing system. In some embodiments, the grid computing system includes a server system which receives processing tasks from one or more client applications, divides the processing tasks into units of work, and assigns the units of work to one or more of the nodes. Dividing a processing task into units of work may involve dividing audio input data into segments defined by natural speech boundaries. A mathematical representation may be created for each segment prior to its distribution on the grid to minimize network traffic. A node in the system may perform heterogeneous units of work concurrently, such as by isolating the execution of each unit of work in an application domain.

Abstract: Methods and apparatus are provided which may be employed to perform speech recognition processing on a grid computing system. In some embodiments, the grid computing system includes a server system which receives processing tasks from one or more client applications, divides the processing tasks into units of work, and assigns the units of work to one or more of the nodes. Dividing a processing task into units of work may involve dividing audio input data into segments defined by natural speech boundaries. A mathematical representation may be created for each segment prior to its distribution on the grid to minimize network traffic. A node in the system may perform heterogeneous units of work concurrently, such as by isolating the execution of each unit of work in an application domain.