Abstract: Systems, methods, and devices for particle separation are provided. A cell sedimentation module may include a housing, which may include a cell sedimentation vessel disposed within the housing, the cell sedimentation vessel having sidewalls, a bottom surface, and a cell collection well formed into the bottom surface. The sedimentation module may include a first flow line configured for input of a cell suspension and output of a supernatant. The sedimentation module may include a second flow line configured for output of a target cell suspension, wherein the cell sedimentation vessel is configured to non-mechanically generate a centripetal flow therein to facilitate separation of the supernatant and the target cell suspension from the cell suspension. In embodiments, the cell sedimentation module may incorporate multiple stacked vessels. In alternative embodiments, the cell sedimentation module may include at least one insert disposed therein to facilitate separation of supernatant and target cell suspension.

Abstract: The present disclosure provides combinatorial fluid switches that allow for the selection of a single fluid flow-path, while controlling multiple flow-paths. The combinatorial fluid switches can be used in various biological systems and processes, included automated cell engineering systems.

Abstract: The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that provide increased cell yield. A cell culture chamber may include a first body forming a first portion of the cell culture chamber. A cell culture chamber may include a second body forming a second portion of the cell culture chamber, the first body and the second body configured to couple together, forming an enclosed volume, wherein a first non-porous, gas-permeable material is disposed on the first body; and wherein the first non-porous, gas-permeable material and the first body are formed together. A second non-porous, gas-permeable material may be disposed on the second body, and the second non-porous, gas-permeable material and the second body may be formed together. The cell culture chamber may further include at least one bubble trap integrally formed with the first body and/or the second body.