Abstract: An insect holder for holding an insect of a particular type, e.g. a forager honey bee, comprises a housing with a chamber adapted to receive an insect of the particular type, an inlet to the chamber through which an insect of the particular type can pass to enter the chamber, a head opening to the chamber adapted to permit the head of an insect of the particular type in the chamber to pass therethrough to the exterior of the housing while retaining the insect in the housing, and retaining means for retaining an insect of the particular type in the chamber with the head of the insect protruding through the head opening to the exterior of the housing, such that the insect is unable to turn around in the chamber. In use, an insect of the particular type enters the chamber via the inlet, and is then retained in the holder by the retaining means. The insect is retained in the chamber, with its head protruding through the head opening of the chamber.