Abstract: A kit of parts and methods for determining the presence and quantity of one or more therapeutic monoclonal antibody drugs and/or therapeutic monoclonal antibody inhibitor drug antibodies in a biological sample, the method including providing a reaction liquid comprising the sample, a first conjugate with the target of the therapeutic monoclonal antibody and a first conjugated moiety and a second conjugate with the target of the therapeutic monoclonal antibody and a second conjugated moiety, followed by detecting the change in spectrophotometric signal when the complex between the therapeutic monoclonal antibody drug, the first conjugate and a the second conjugate forms.

Abstract: The present invention relates to a kit of parts and methods for determining the presence and quantity of one or more TNF-? inhibitor drugs and/or anti-TNF-? inhibitor drug antibodies in one or more biological samples each comprising less than 200 ?l, the method comprising the steps of providing a reaction liquid comprising the sample, a first TNF-? conjugate comprising TNF-? and a first conjugated moiety and a second TNF-? conjugate comprising TNF-? and a second conjugated moiety, said second moiety being capable of generating or ameliorating a detectable signal in the presence of a molecular complex comprising a TNF-? inhibitor, followed by detecting the change in signal when the complex between the TNF-? inhibitor drug, the first TNF-? conjugate and a the second TNF-? conjugate forms.

Abstract: Methods and kits-of-parts for determining the quantity of HbA1c relative to the concentration of haemoglobin in a blood sample which includes less than 200 ?l of blood, the method including the steps of adding the fluorophore to the sample and measuring the fluorescence at one or more time points within the time interval, at which the change in fluorescence over time is >0, followed by measurements of haemoglobin by adding a haemoglobin-binding agent and measuring the change in transmission at approximately 570 nm and comparing the obtained results with an internal standard.

Abstract: The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3? part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5? part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3? part that is substantially homolog to a fourth part of the target sequence and a 5? part that is substantially complementary to a third part of the target sequence.

Abstract: The invention relates to a reporter unit for detecting a target molecule comprising at least one component attached to a target molecule specific probe, wherein said at least one component is liberated from said probe by the activity of a first enzyme thereby making the at least one component available as a substrate for a second enzyme which employs the substrate in a polymerization reaction to obtain a detectable structure.

Abstract: The present invention relates to a device and a method for clustering and subsequently distributing a plurality of paramagnetic particles in a small volume liquid in a chamber, said chamber having a first wall and an opposite second wall, said method comprising the steps of a) providing a liquid comprising the plurality of paramagnetic particles, b) subjecting the paramagnetic particles to a first magnetic field by means of a first field generating means having first and second mutually spaced opposite poles and defining a first pole axis extending between the poles, the field generating means being arranged relative to the first wall so that the first pole axis (a1) forms an angle (v1) of less than 60° with the first wall, c) moving the magnetic field in a first moving direction having a movement component in the direction of the first pole axis, or, alternatively, changing the polarity of the magnetic field one or more times, and, d) removing the magnetic field.

Abstract: The present invention relates to a device and a method for quantitative detecting of the presence or absence of a target analyte in a liquid sample having a volume of less than 200 ?l, the device comprising a reaction chamber in the form of a capillary channel, a first part comprising a sample inlet for the introduction of a sample containing an analyte, and a discharge outlet for the discharge of waste products; a second part comprising means for detection of the target analyte, and a solution inlet for introduction of washing solutions and reaction mixtures; and means for transferring an immobilized analyte from the first part to the second part of the chamber and vice versa, where the first and second parts are separated such that other liquid sample material may not enter the second part of the chamber and such that light may not be transferred from the first part of the chamber to the detector part of the second part of the chamber.

Abstract: The present invention relates to a device and a method for quantitative detecting of the presence or absence of a target analyte in a liquid sample, the device comprising a reaction chamber having a volume of less than 200 ?l comprising an immobilization matrix capable of capturing the analyte, said immobilization matrix, preferably comprising a particulate magnetic material, having a size distribution that is at least bimodal.

Abstract: The present invention relates to a device for separating a suspension into a liquid phase and a retentate phase. The device comprises a separation chamber comprising an application zone and a hydrophilic filter material. The separation chamber is connected to a first capillary channel, where the connecting junction between the separation chamber and the first capillary channel comprise a physical barrier preventing flow of residue retentate from a lower part of the chamber into the first capillary channel. The invention further relates to a method for separating a liquid sample consisting of less than 200 ?l suspension, into a retentate phase comprising the suspended matter, and a liquid phase substantially free of suspended matter.

Abstract: The present invention relates generally to a signal amplification method for a SAW resonator microsensor for analyzing test samples, containing target analyte including proteins and nucleic acids. The method comprising the steps: reacting the analyte in a sample with a first molecular recognition component and a second molecular recognition component linked to at least one nanoparticle; and adding an enhancement solution comprising silver ions and/or gold ions, and a reducing agent, whereby the silver ions and/or gold ions are reduced to metallic silver and/or gold which is deposited onto the surface of the at least one nanoparticle; wherein the mass increase of the at least one nanoparticle is detected by a SAW sensor.

Abstract: The invention relates to a reporter unit for detecting a target molecule comprising at least one component attached to a target molecule specific probe, wherein said at least one component is liberated from said probe by the activity of a first enzyme thereby making the at least one component available as a substrate for a second enzyme which employs the substrate in a polymerization reaction to obtain a detectable structure.

Abstract: The present invention relates generally to a signal amplification method for a SAW resonator microsensor for analyzing test samples, containing target analyte including proteins and nucleic acids. The invention relates to at least one surface acoustic wave resonator unit comprising a plurality of three-dimensional interdigital transducer electrode (IDTE) and reflector micro channels located on a piezoelectric substrate surface. The invention further relates to a change of solid/liquid volume ratio in said three-dimensional micro channels; said changes of the liquid/solid volume ratio can be directed correlated to the target analyte concentration in a test sample.

Abstract: The present invention relates generally to methods and compositions for analyzing test samples containing target analytes including proteins and nucleic acids. The invention uses a surface acoustic wave sensor in combination with a hydrogel to obtain an ultra sensitive non-fluorescent detection system.

Abstract: The present invention relates generally to methods and compositions for analyzing test samples containing target analytes including proteins and nucleic acids. The invention uses a surface acoustic wave sensor in combination with a hydrogel to obtain an ultra sensitive non-fluorescent detection system.

Abstract: The present invention relates generally to methods and compositions for analyzing binding molecules including proteins and nucleic acid molecules. In addition the invention relates to the use of surface acoustic wave sensors that rely on non-fluorescent detection system consisting of a sensor using propagation of surface acoustic waves for detection of a wide variety of biological-based assays.

Abstract: This invention relates to a method wherein special adaptors and primers are used for single stranded cDNA amplification. The method comprises contacting RNA with an anchored cDNA synthesis primer which can anneal to RNA, a suitable enzyme which possesses reverse transcriptase activity, and a special designed adaptor which can efficiently be ligated to the 3′ end of single stranded cDNA by T4 DNA ligase. Processes are provided for using specific primers annealing to the adaptors in the 5′ and 3′-end of the single stranded cDNA for PCR amplifying the cDNA. The invention describes the use of this approach for generation of probes starting from low amounts of RNA to be used e.g. in hybridization towards micro-arrays.

Abstract: The present invention relates generally to methods and compositions for analyzing binding molecules including proteins and nucleic acid-molecules. In addition the invention relates to the use of microsensors that rely on non-fluorescent detection system consisting of a sensor using microscopic flexible mechanical structures such as micro-cantilevers or micro-membranes integrated into a microscopic chambers for detection of a wide variety of biological-based assays.

Abstract: The invention relates to a method for enzyme stabilization. A method for improved reverse transcription at high temperatures is provided, wherein a thermostable heat shock protein (HSPs) stabilizes a reverse transcriptase, as well as reduces the RNase H activity of said reverse transcriptase. The present invention thus relates to a stabilizing agent, that prevents thermal denaturing and enhances thermostability of a reverse transcriptase. The invention further relates to a method of producing a polypeptide complex consisting of a Chaperonin and a Moloney murine leukemia virus (MMVL) reverse transcriptase, characterized by having enhanced thermostability as well as reduced RNase H activity, compared to a (MMVL) reverse transcriptase alone. The invention further relates to a kit for the preparation of cDNA from mRNA, comprising either both stabilizing agent and reverse transcriptase or the polypeptide complex of the invention.