Abstract: The present application describes an optimized medium for growth of mammalian cells as well as polypeptide production. The cell culture medium is characterized by a Sow ratio of sodium to potassium ions, it further relates to the method of producing polypeptides using such cell culture media. Sn another aspect, the method of polypeptide production can also comprise a temperature shift and/or a pH-shift to further optimize growth and product yield.

Abstract: The present application describes an optimized medium for growth of mammalian cells as well as polypeptide production. The cell culture medium is characterized by a Sow ratio of sodium to potassium ions, it further relates to the method of producing polypeptides using such cell culture media. Sn another aspect, the method of polypeptide production can also comprise a temperature shift and/or a pH-shift to further optimize growth and product yield.

Abstract: Mammalian cells which require contact with cell surface proteins for activation, differentiation and/or proliferation are cultured in contact with an immobilized mammalian cell, with an immobilized membrane vesicle from a mammalian cell or with immobilized membrane vesicles from at least two mammalian cell populations. The mammalian cell or membrane vesicle can be modified with a first partner of a biological binding pair, and immobilized on a solid carrier via a second partner of the biological binding pair. Culturing can be with a first immobilized membrane vesicle and subsequently with a second immobilized membrane vesicle. The membrane vesicle contains part of the natural surface of a mammalian cell that includes a signal triggering surface protein. The membrane vesicle can be immobilized via a binding partner of a cell surface molecule.

Abstract: In a device for connecting apparatus for withdrawing ultrapure liquids from containers, the connection plate 4 is provided with three bores 5, 6, 7, of which two bores 5, 6 each extend through a socket 8 for vertical pipes. The top plate 9 has an integrated valve body 23 for accommodating at least two valve heads 2, 3 and three bores 10, 11, 12, which are congruent with the bores 5, 6, 7 of the connection plate 4. The bore 12 extends through the valve body 23, and the two other bores 10, 11 terminate, at one end thereof, in pipe sockets 29, 30, which project into the bores 5, 6 of the connection plate 4, and, at the other end thereof, in channels 31, 32, 33, 34 for the ultrapure liquids, which are arranged in the valve body 23 and are interrupted by the valve heads 2, 3.

Abstract: The present application describes an optimized medium for growth of mammalian cells as well as polypeptide production. The cell culture medium is characterized by a Sow ratio of sodium to potassium ions, it further relates to the method of producing polypeptides using such cell culture media. Sn another aspect, the method of polypeptide production can also comprise a temperature shift and/or a pH-shift to further optimize growth and product yield.