Abstract: A novel process for preparing non-dusting, enzyme-containing particles, e.g. for introduction into washing compositions, comprising homogeneously mixing an amount of an enzyme in dry or substantially dry form to give the desired enzyme activity in the particles when formed, about 20% to about 60% of a hydrophilic organic cohesive compatible with the enzyme, about 10% to about 60% of a building agent suitable for use in enzyme compositions, and a sufficient amount of water, if necessary, to give a moisture content in the particles, when formed, of from 5% to 15%, mechanically dividing the mixture obtained into particles of the desired size, and coating the particles to prevent loss of moisture and the novel enzyme particles produced thereby as well as washing compositions and pharmaceutical compositions containing said enzyme particles.