Abstract: The present invention provides a method for restoring immune tolerance in vivo. The invention relates to the use of a recombinant gene encoding auto-antigens or parts thereof for restoring immune tolerance to the auto-antigens in vivo, under transcriptional control of polyomaviral early and late promoters. In a preferred embodiment, the invention relates to the use of recombinant polyomaviral gene delivery vector particles, such as simian virus 40 (SV40) viral vector particles encoding one or multiple auto-antigens or parts thereof under transcriptional control of the SV40 early and late promoter, for restoring immune tolerance to the auto-antigens in vivo. The invention also relates to compositions comprising recombinant genes or polyomaviral vectors and uses thereof as treatment for degenerative or dystrophic diseases.

Abstract: The disclosure is in the field of cell therapy, more in particular, stem cell transplantation therapy. The disclosure provides methods and compositions for improving the efficacy of stem cell transplantation therapy by reducing the inflammatory activity of a stem cell transplant. More in particular, the disclosure provides a method for preparing a stem cell transplant with reduced inflammatory activity comprising a step of suspending a composition comprising stem cells in a fibrinogen-depleted plasma and/or in a fibrinogen and C-reactive protein-depleted plasma.

Abstract: The disclosure is in the field of cell therapy, more in particular, stem cell transplantation therapy. The disclosure provides methods and compositions for improving the efficacy of stem cell transplantation therapy by reducing the inflammatory activity of a stem cell transplant. More in particular, the disclosure provides a method for preparing a stem cell transplant with reduced inflammatory activity comprising a step of suspending a composition comprising stem cells in a fibrinogen-depleted plasma and/or in a fibrinogen and C-reactive protein-depleted plasma.

Abstract: The disclosure is in the field of cell therapy, more in particular, stem cell transplantation therapy. The disclosure provides methods and compositions for improving the efficacy of stem cell transplantation therapy by reducing the inflammatory activity of a stem cell transplant. More in particular, the disclosure provides a method for preparing a stem cell transplant with reduced inflammatory activity comprising a step of suspending a composition comprising stem cells in a fibrinogen-depleted plasma and/or in a fibrinogen and C-reactive protein-depleted plasma.

Abstract: The present invention provides a method for restoring immune tolerance in vivo. The invention relates to the use of a recombinant gene encoding auto-antigens or parts thereof for restoring immune tolerance to the auto-antigens in vivo, under transcriptional control of polyomaviral early and late promoters. In a preferred embodiment, the invention relates to the use of recombinant polyomaviral gene delivery vector particles, such as simian virus 40 (SV40) viral vector particles encoding one or multiple auto-antigens or parts thereof under transcriptional control of the SV40 early and late promoter, for restoring immune tolerance to the auto-antigens in vivo. The invention also relates to compositions comprising recombinant genes or polyomaviral vectors and uses thereof as treatment for degenerative or dystrophic diseases.

Abstract: The disclosure is in the field of cell therapy, more in particular, stem cell transplantation therapy. The disclosure provides methods and compositions for improving the efficacy of stem cell transplantation therapy by reducing the inflammatory activity of a stem cell transplant. More in particular, the disclosure provides a method for preparing a stem cell transplant with reduced inflammatory activity comprising a step of suspending a composition comprising stem cells in a fibrinogen-depleted plasma and/or in a fibrinogen and C-reactive protein-depleted plasma.

Abstract: Polyoma viral vector production cell line comprising a heterologous polynucleotide sequence that is capable of being transcribed into an RNA sequence that is capable of folding into double stranded RNA of at least 50 base pairs in length, methods of producing said cell line, uses thereof and recombinant polyoma viral vectors and nucleic acid sequences relating thereto.

Abstract: A method for treating viral infections in vivo in subjects in need of such treatment by applying heat to affected body tissue, use of an apparatus to provide heated moist gas or heated liquids to affected body tissue that is infected with a virus and apparatuses capable of providing heat to a body part that is infected with a virus.

Abstract: This invention describes novel DNA sequences that function as promoters of transcription of associated nucleotide sequences. More specifically, this invention describes DNA sequences conferring constitutive expression to an associated nucleotide sequence. The invention also describes recombinant sequences containing such promoter sequences. The said recombinant DNA sequences may be used to create transgenic plants, but especially plants expressing a nucleotide sequence of interest at all times and in most tissues and organs.

Abstract: The present invention relates to a method to confer resistance or tolerance to more than one virus from the group consisting of furovirus, potyvirus, tospovirus, and cucomovirus, using sense and antisense RNA fragments of a sequence from their genomes. The sense and antisense RNA fragments are capable of pairing and forming a double-stranded RNA molecule, thereby reducing expression of the viral genome.

Abstract: The invention relates to a nucleic acid and the encoded plant resistance protein which upon tobamovirus infection interacts with the 30K tobamovirus movement protein to protect the plant against the spread of the infection. Simultaneous expression of the resistance protein and a 30K movement protein, wherein expression of at least one of them is controlled by a pathogen-inducible promoter, can be used in a general method of protecting plants from the spread of a pathogen infection.

Abstract: Recombinant DNA constructs comprising a DNA coding for transcription into an RNA sequence of tospoviruses or into RNA sequences related thereto, the use of such DNA constructs to transform plants having reduced susceptibility to tospovirus infection and probes for the isolation of tospovirus or diagnosis of plant tospovirus diseases.

Abstract: Plant transformation vectors comprising a polynucleotide effective to render resisitance or tolerance to infection by a tospovirus, and a microbiological process for making virus tolerant or resistant plants are provided herein.

Abstract: Recombinant DNA constructs comprising a DNA coding for transcription into an RNA sequence of tospoviruses or into RNA sequences related thereto, the use of such DNA constructs to transform plants having reduced susceptibility to tospovirus infection and probes for the isolation of tospovirus or diagnosis of pant tospovirus diseases.

Abstract: DNA constructs encoding an RNA molecule capable of interacting with an RNA dependent RNA polymerase encoded for by a virus when invading a plant such that either an eliciting element or a plus sense RNA is produced as a consequence of the interaction with the RNA dependent RNA polymerase encoded by the said invading virus, whereby any produced plus sense RNA molecule is capable of encoding for an eliciting element, plants containing such constructs and processes for obtaining such plants.

Abstract: A nucleotide sequence comprising a transcriptional regulatory sequence and a sequence contiguous therewith and under the transcriptional control thereof, which contiguous sequence encodes an RNA which consists of a plurality of sub-sequences, characterized in that at least two of the sub-sequences have the sequences of viral RNAs and the RNA contains at least one translational stop codon located upstream of the 3' terminal sub-sequence. It is preferred that at least one of the sub-sequences is in an anti-sense configuration with respect to virus RNA, and that the contiguous sequence encodes mRNA. The invention also includes, inter alia, the use of such a sequence in the generation of virus resistant or tolerant plants, and such plants comprising the sequence.

Abstract: Recombinant Impatiens Necrotic Spot Virus (INSV) DNA constructs comprising an INSV DNA coding for transcription into INSV RNA sequences or into RNA sequences related thereto, the use of such DNA constructs to transform plants having reduced susceptibility to INSV infection and probes for the isolation of INSV or diagnosis of plant INSV related diseases.