Abstract: The present invention discloses a genetically engineered Kluyveromyces sp. yeast cell comprising at least a genetic modification that inactivates or deletes a nucleic acid sequence encoding for transcription factor SEQ ID No. 2, particularly Haal. The genetically engineered yeast cell according to this invention has an improvement of lactic acid tolerance, lactic acid production or a combination thereof as compared to the parental.

Abstract: The present invention discloses a genetically engineered Kluyveromyces sp. yeast strain that is capable of producing lactic acid from carbon source selected from glucose, fructose, sucrose or a mixture thereof wherein the genetically engineered yeast comprises at least one heterologous DNA cassette that confers production of a protein functioning as a fructose importer. The genetically engineered yeast strain according to this invention has an improvement of fructose utilization and use fructose as a faster rate than conventional strain, allowing for shorter fermentation times and improved economics.

Abstract: The present invention relates to a novel mutant strain Aspergillus aculeatus E14-292 and a genetic modification process of said strain, wherein the mutant strain according to this invention can produce cellulase and xylanase more than the BCC199 (wild type). Moreover, the obtained enzymes can be used to digest the pretreatment bagasse to further produce sugars effectively.

Abstract: The present invention relates to a novel mutant strain Aspergillus aculeatus E14-292 and a genetic modification process of said strain, wherein the mutant strain according to this invention can produce cellulase and xylanase more than the BCC199 (wild type). Moreover, the obtained enzymes can be used to digest the pretreatment bagasse to further produce sugars effectively.

Abstract: This invention relates to a process for producing lactic acid or its salts that can be performed easily, reduce complicated steps, and provide high lactic acid yield and high productivity, wherein said process comprising of the following steps: (a) cultivating thermotolerance Bacillus genus bacteria to obtain a seed culture; (b) increasing cell number of bacteria by inoculating the seed culture contained from step (a) into a fermenter containing an initial carbon source under an aerobic condition; (c) fermenting the seed culture obtained from step (b) in the fermenter under a microaerobic condition to obtain lactic acid or its salts; wherein the step (b) comprising at least one addition of the carbon source under any one of the following conditions, where are independent to each other, to increase a concentration of the carbon source: when the concentration of the carbon source in the fermenter reduces to 50 % or less comparing to the initial concentration.

Abstract: This invention relates to a process for producing lactic acid or its salts that can be performed easily, reduce complicated steps, and provide high lactic acid yield and high productivity, wherein said process comprising the following steps: (a) cultivating thermotolerance Bacillus genus bacteria to obtain a seed culture; (b) increasing cell number of bacteria by inoculating the seed culture obtained from step (a) into a fermenter containing an initial carbon source under an aerobic condition; (c) fermenting the seed culture obtained from step (b) in the fermenter under a microaerobic condition to obtain lactic acid or its salts; wherein the step (b) comprising at least one addition of the carbon source under any one of the following conditions, which are independent to each other, to increase a concentration of the carbon source: —when the concentration of carbon source in the fermenter reduces to 50% or less comparing to the initial concentration.

Abstract: This invention relates to a fermentation process for producing D-lactic acid or its salts. The said process comprises the following steps: cultivating of Sporolactobacillus laevolacticus bacteria strain by fermentation with sugarcane juice in order to obtain the seed culture; fermenting of the obtained seed culture in sugarcane juice, wherein the cultivation step is operated for the time that the final concentration of Sporolactobacillus laevolacticus strain in the cultivation step is in a range of 400 to 1,600 mg of dry cell per liter. The said process according to the invention can provide high productivity and yield of the D-lactic acid with a high optical purity. Moreover, the said process can be easily done and reduces the complicate steps.

Abstract: The present invention relates to a novel Bacillus aerolacticus BC-001 species and a method for producing lactic acid or its salts using said bacteria, wherein the novel bacteria species can produce L-lactic acid or its salts with high optical purity from carbohydrate carbon sources, including oligosaccharide, disaccharide and monosaccharide, wherein said bacteria can grow well under an aerobic condition, tolerate to high temperature in a range of 45 to 60° C.

Abstract: This invention relates to a process for producing lactic acid or its salts that can be performed easily, reduce complicated steps, and provide high lactic acid yield and high productivity, wherein said process comprising the following steps: (a) cultivating thermotolerance Bacillus genus bacteria to obtain a seed culture; (b) increasing cell number of bacteria by inoculating the seed culture obtained from step (a) into a fermenter containing an initial carbon source under an aerobic condition; (c) fermenting the seed culture obtained from step (b) in the fermenter under a microaerobic condition to obtain lactic acid or its salts; wherein the step (b) comprising at least one addition of the carbon source under any one of the following conditions, which are independent to each other, to increase a concentration of the carbon source: —when the concentration of carbon source in the fermenter reduces to 50% or less comparing to the initial concentration.

Abstract: The present invention disclosed a bio-based polymer additive, its preparation process and a biodegradable polymer composition comprising the said bio-based polymer additive for use in manufacturing of biodegradable plastic. The said additive is prepared from the biomass of broken microorganism cell such as microalgae, yeast or other microorganisms. In particular, the bio-based polymer additive is for enhancing rheological properties and/or biodegradability of a polymer. In particular, the additive is for use as a pigment.

Abstract: The present invention disclosed a bio-based polymer additive, its preparation process and a biodegradable polymer composition comprising the said bio-based polymer additive for use in manufacturing of biodegradable plastic. The said additive is prepared from the biomass of broken microorganism cell such as microalgae, yeast or other microorganisms. In particular, the bio-based polymer additive is for enhancing rheological properties and/or biodegradability of a polymer. In particular, the additive is for use as a pigment.

Abstract: The present invention provides the new method for extracting lutein from natural materials wherein the said method comprises of modification of natural lutein ester in the natural materials to free lutein and/or low molecular weight lutein ester, extraction of the said natural materials with supercritical fluid at the optimal conditions. The said method yields high amount of crude lutein with high purity due to the mild condition used for extraction. Therefore, no degradation of the desired product is occurred. The crude lutein can be further purified with chromatography in order to obtain the highly purified lutein. The method according to this invention can be applied to the extraction of xanthophylls or others beside lutein.

Abstract: The present invention provides the new method for extracting lutein from natural materials wherein the said method comprises of modification of natural lutein ester in the natural materials to free lutein and/or low molecular weight lutein ester, extraction of the said natural materials with supercritical fluid at the optimal conditions. The said method yields high amount of crude lutein with high purity due to the mild condition used for extraction. Therefore, no degradation of the desired product is occurred. The crude lutein can be further purified with chromatography in order to obtain the highly purified lutein. The method according to this invention can be applied to the extraction of xanthophylls or others beside lutein.