Abstract: A method for oxidizing an alkyl, including a) contacting the alkyl with an aqueous solution comprising a microorganism where the microorganism has a reduced fatty acid degradation capacity compared to its wild type, wherein the fatty acid degradation capacity is reduced by deletion, inhibition or inactivation of a gene encoding an enzyme involved in the ?-oxidation pathway; and the microorganism expresses a recombinant alkane oxidase, and b) contacting the aqueous solution from a) with a water-immiscible organic solvent.

Abstract: A method for oxidizing an alkyl, including a) contacting the alkyl with an aqueous solution comprising a microorganism where the microorganism has a reduced fatty acid degradation capacity compared to its wild type, wherein the fatty acid degradation capacity is reduced by deletion, inhibition or inactivation of a gene encoding an enzyme involved in the ?-oxidation pathway; and the microorganism expresses a recombinant alkane oxidase, and b) contacting the aqueous solution from a) with a water-immiscible organic solvent.

Abstract: The present invention relates to a microbial cell for producing at least one L-amino acid from at least one C1-C4 alkane, wherein the cell comprises: (i) an increased expression relative to the wild type cell of Enzyme E1 capable of converting the alkane to a corresponding 1-alkanol; (ii) an increased expression relative to the wild type cell of Enzyme E2 capable of converting the 1-alkanol of (i) to a corresponding aldehyde; and either (iii) (A) an increased expression relative to the wild type cell of Enzyme E3 capable of converting the aldehyde of (ii) to a corresponding alkanoic acid; and a wild-type level expression of Enzyme E4 or an increased expression relative to the wild type cell of Enzyme E4 capable of converting the alkanoic acid of (iii) to a corresponding fatty acyl thioester; or ?(B) an increased expression relative to the wild type cell of Enzyme E5 capable of converting the aldehyde of (ii) to a corresponding fatty acyl thioester; ?and (iv) an increased expression relative to the wi

Abstract: The invention relates to a process for preparing an ?,?-alkanediol comprising the steps of a) reacting an alkanoic acid with an alkanol to give an ester, b) oxidizing at least one terminal carbon atom of the ester by contacting with a whole-cell catalyst, which expresses an alkane hydroxylase, in aqueous solution and in the presence of molecular oxygen, to give an oxidized ester, c) hydrogenating the oxidized ester to form the alkanediol and alkanol, and d) removing the alkanol by distillation, forming a reaction mixture depleted with respect to the alkanol, and recycling the alkanol in step b).

Abstract: The present invention relates to a method of preparing at least one rhamnolipid comprising: contacting a recombinant cell with a medium containing a carbon source; and culturing the cell under suitable conditions for preparation of the rhamnolipid from the carbon source by the cell, wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E1, E2 and E3, wherein the enzyme E1 is an ?/? hydrolase, the enzyme E2 is a rhamnosyltransferase I and the enzyme E3 is a rhamnosyl-transferase II, and wherein the carbon source is a C4 molecule.

Abstract: The present invention relates to a method of preparing at least one rhamnolipid comprising: contacting a recombinant cell with a medium containing a carbon source; and culturing the cell under suitable conditions for preparation of the rhamnolipid from the carbon source by the cell, wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E1, E2 and E3, wherein the enzyme E1 is an ?/? hydrolase, the enzyme E2 is a rhamnosyltransferase I and the enzyme E3 is a rhamnosyl-transferase II, and wherein the carbon source is a C4 molecule.

Abstract: The present invention relates to a method comprising the method steps A) Providing at least one compound of the general formula I) where X=divalent organic radical comprising 1 to 19 carbon atoms B) Contacting the compound of the general formula I) with an enzyme E1 selected from the group esterases, lipases and lactonases, characterized in that method step B) is carried out in the presence of at least one aliphatic alcohol comprising 1 to 6 carbon atoms.

Abstract: The invention relates to a process for preparing an ?,?-alkanediol comprising the steps of a) reacting an alkanoic acid with an alkanol to give an ester, b) oxidizing at least one terminal carbon atom of the ester by contacting with a whole-cell catalyst, which expresses an alkane hydroxylase, in aqueous solution and in the presence of molecular oxygen, to give an oxidized ester, c) hydrogenating the oxidized ester to form the alkanediol and alkanol, and d) removing the alkanol by distillation, forming a reaction mixture depleted with respect to the alkanol, and recycling the alkanol in step b).

Abstract: The present invention relates to a method comprising the steps a) providing an alkane or 1-alkanol, b) contacting said alkane or 1-alkanol in an aqueous solution with a cytochrome P450 alkane hydroxylase from the CYP153 family and oxygen for at least 3 hours.

Abstract: The present invention relates to a microorganism having a reduced fatty acid degradation capacity and expressing a recombinant alkane oxidase, a method for oxidizing an alkyl, comprising a contacting the alkyl with an aqueous solution comprising the inventive cell.

Abstract: The present invention relates to method for oxidising an alkene comprising contacting said alkene with an AlkB-type oxidoreductase in the presence of oxygen, and a use of an AlkB-type oxidoreductase, preferably AlkB from Pseudomonas putida GPO1 or a variant thereof, for oxidising an alkene to an alcohol and/or acid.

Abstract: An embodiment of the invention relates to a single photon emission system having a proximal end, a distal end, and a single photon emitter located between the proximal end and the distal end; wherein the single photon emission system is adapted to guide optical pump radiation, which is inputted at the proximal end to optically excite the single photon emitter, along a predefined direction that runs from the proximal end to the distal end; and wherein single photons emitted by said single photon emitter, are guided along said predefined direction to the distal end.

Abstract: An embodiment of the invention relates to a single photon emission system having a proximal end, a distal end, and a single photon emitter located between the proximal end and the distal end; wherein the single photon emission system is adapted to guide optical pump radiation, which is inputted at the proximal end to optically excite the single photon emitter, along a predefined direction that runs from the proximal end to the distal end; and wherein single photons emitted by said single photon emitter, are guided along said predefined direction to the distal end.