Patents by Inventor Philip K. Hsieh

Philip K. Hsieh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Production of biologically active insulin-like growth factor I from high expression host cell systems
    Patent number: 5158875
    Abstract: A novel method for refolding reduced IGF-I is provided in accordance with the present invention. The method is carried out by providing additional positive charge at the amino terminal end of the IGF-I molecule by the presence of a short leader sequence. The additional positive charge on the met-end of the IGF-I molecule has been found to enable recombinant IGF-I to refold simply by stirring solubilized inclusion body protein for between 2-16 hours, or overnight. The present invention also provides a novel fusion protein intermediate of IGF-I attached to the positively charged leader sequence.
    Type: Grant
    Filed: August 25, 1989
    Date of Patent: October 27, 1992
    Assignee: Amgen Inc.
    Inventors: James A. Miller, Philip K. Hsieh, Larry B. Tsai