Patents by Inventor Philip M. Lester

Philip M. Lester has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Purification of microbial cell fermentation homogenates
    Patent number: 7579448
    Abstract: A method for purifying a desired heterologous polypeptide from microbial fermentation broth or homogenate in which it is produced and solubilized is described. This method involves adding to the broth or homogenate an effective amount of a solution of 6,9-diamino-2-ethoxyacridine lactate (ethacridine lactate) to precipitate host cell impurities under conditions wherein the majority of polypeptide remains soluble, and separating the desired polypeptide from the broth or homogenate. The broth or homogenate containing the ethacridine lactate and polypeptide is also disclosed.
    Type: Grant
    Filed: December 12, 2006
    Date of Patent: August 25, 2009
    Assignee: Genentech, Inc.
    Inventors: Philip M. Lester, Josefine Persson
  • Purification of polypeptides
    Patent number: 7169908
    Abstract: A method for purifying a desired heterologous polypeptide from microbial fermentation broth or homogenate in which it is produced and solubilized is described. This method involves adding to the broth or homogenate an effective amount of a solution of 6,9-diamino-2-ethoxyacridine lactate (ethacridine lactate) to precipitate host cell impurities under conditions wherein the majority of polypeptide remains soluble, and separating the desired polypeptide from the broth or homogenate. The broth or homogenate containing the ethacridine lactate and polypeptide is also disclosed.
    Type: Grant
    Filed: January 8, 2004
    Date of Patent: January 30, 2007
    Assignee: Genentech, Inc.
    Inventors: Philip M. Lester, Josefine Persson
  • Protein purification
    Patent number: 6870034
    Abstract: A method for purifying proteins by Protein A chromatography is described which comprises removing contaminants by washing the solid phase with various intermediate wash buffers.
    Type: Grant
    Filed: February 3, 2003
    Date of Patent: March 22, 2005
    Assignee: Genentech, Inc.
    Inventors: Timothy N. Breece, Robert L. Fahrner, Jeffrey R. Gorrell, Kathlyn Pham Lazzareschi, Philip M. Lester, David Peng
  • Protein purification
    Publication number: 20030153735
    Abstract: A method for purifying proteins by Protein A chromatography is described which comprises removing contaminants by washing the solid phase with various intermediate wash buffers.
    Type: Application
    Filed: February 3, 2003
    Publication date: August 14, 2003
    Applicant: GENENTECH, INC.
    Inventors: Timothy N. Breece, Robert L. Fahrner, Jeffrey R. Gorrell, Kathlyn Pham Lazzareschi, Philip M. Lester, David Peng