Patents by Inventor Philip Richard Buzby
Philip Richard Buzby has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9012144Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.Type: GrantFiled: January 18, 2011Date of Patent: April 21, 2015Assignee: Fluidigm CorporationInventors: Stanley Lapidus, Philip Richard Buzby, Timothy Harris
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Patent number: 8367377Abstract: Methods of the invention comprise methods and devices for nucleic acid sequence determination. Generally, the invention relates to preparing a substrate for sequencing a target nucleic acid.Type: GrantFiled: November 16, 2009Date of Patent: February 5, 2013Assignee: Helicos Biosciences CorporationInventors: Timothy Harris, Philip Richard Buzby, Mirna Jarosz, James Joseph Dimeo, Jaime Gill
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Publication number: 20110152114Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.Type: ApplicationFiled: January 18, 2011Publication date: June 23, 2011Applicant: HELICOS BIOSCIENCES CORPORATIONInventors: Stanley Lapidus, Philip Richard Buzby, Timothy Harris
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Publication number: 20100227321Abstract: Methods of the invention comprise methods and devices for nucleic acid sequence determination.Type: ApplicationFiled: November 16, 2009Publication date: September 9, 2010Applicant: Helicos Biosciences CorporationInventors: Timothy Harris, Philip Richard Buzby, Mirna Jarosz, James Joseph DiMeo, Jaime Gill
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Patent number: 7767805Abstract: The invention provides nucleotide analogs and methods of using them in sequencing reactions.Type: GrantFiled: May 2, 2008Date of Patent: August 3, 2010Assignee: Helicos Biosciences CorporationInventor: Philip Richard Buzby
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Patent number: 7635562Abstract: Methods of the invention comprise methods and devices for nucleic acid sequence determination. Generally, the invention relates to preparing a substrate for sequencing a target nucleic acid.Type: GrantFiled: May 25, 2005Date of Patent: December 22, 2009Assignee: Helicos BioSciences CorporationInventors: Timothy Harris, Philip Richard Buzby, Mirna Jarosz, James Joseph DiMeo, Jaime Gill
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Publication number: 20090061439Abstract: The invention provides nucleotide analogs and methods of using them in sequencing reactions.Type: ApplicationFiled: May 2, 2008Publication date: March 5, 2009Applicant: HELICOS BIOSCIENCES CORPORATIONInventor: Philip Richard Buzby
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Patent number: 7482120Abstract: The invention provides methods and compositions for improving the fidelity of a sequencing-by-synthesis reaction by using a nucleotide derivative that forms a hydrogen bond with a complementary nucleotide on a template, but fails to form a phosphodiester bond with the 3? hydroxyl group of a primer under conditions otherwise suitable for a polymerization reaction; thereby blocking incorporation of a mismatched nucleotide.Type: GrantFiled: January 28, 2005Date of Patent: January 27, 2009Assignee: Helicos Biosciences CorporationInventor: Philip Richard Buzby
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Publication number: 20080076123Abstract: Described herein is a mutant polymerase, specifically the Klenow exo? polymerase in which the proline at amino acid position 680 is replaced by a glycine (P680G), and its use in sequencing a single polynucleotide template using single molecule sequencing.Type: ApplicationFiled: September 27, 2006Publication date: March 27, 2008Applicant: Helicos BioSciences CorporationInventor: Philip Richard Buzby
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Patent number: 7220549Abstract: The invention provides methods for sequencing a nucleic acid comprising stabilizing a primer/target nucleic acid duplex on a substrate. Methods of the invention generally contemplate the use of a dual-anchored primer/target nucleic acid duplex, or a stabilizing molecule in a single molecule sequencing reaction.Type: GrantFiled: December 30, 2004Date of Patent: May 22, 2007Assignee: Helicos Biosciences CorporationInventor: Philip Richard Buzby
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Patent number: 7169560Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.Type: GrantFiled: May 24, 2004Date of Patent: January 30, 2007Assignee: Helicos Biosciences CorporationInventors: Stanley N Lapidus, Philip Richard Buzby, Timothy Harris
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Patent number: 6437141Abstract: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: April 2, 2001Date of Patent: August 20, 2002Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Publication number: 20010020098Abstract: A cyanine dye having the formula 1Type: ApplicationFiled: April 2, 2001Publication date: September 6, 2001Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, David George Ahern, Mark Norman Bobrow
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Patent number: 6224644Abstract: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: November 24, 1999Date of Patent: May 1, 2001Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Patent number: 6204389Abstract: A cyanine dye having the formula wherein R1--R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: November 24, 1999Date of Patent: March 20, 2001Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Patent number: 6197956Abstract: A cyanine dye having the formula wherein R1-R8 are each independently selected from a group consisting of hydrogen, C1-C6 alkyl group, and C0-C4 alkyl group having a hydrophilic substituent thereon. R11 and R12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: November 24, 1999Date of Patent: March 6, 2001Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow
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Patent number: 6114350Abstract: A cyanine dye having the formula ##STR1## wherein R.sub.1 -R.sub.8 are each independently selected from a group consisting of hydrogen, C.sub.1 -C.sub.6 alkyl group, and C.sub.0 -C.sub.4 alkyl group having a hydrophilic substituent thereon. R.sub.11 and R.sub.12 are chosen to include a free or protected thiol, amine or hydroxyl substituent capable of reacting with a target molecule through a nucleophilic displacement mechanism. The dye is useful in labeling a variety of target molecules. Processes are described for synthesizing suitable heterocyclic and indole derivatives as precursors for the aforementioned cyanine dyes.Type: GrantFiled: April 19, 1999Date of Patent: September 5, 2000Assignee: NEN Life Science Products, Inc.Inventors: Malcolm Harry Randall, Philip Richard Buzby, Thomas Joseph Erickson, Joseph David Trometer, Joseph John Miller, Jr., David George Ahern, Mark Norman Bobrow