Abstract: There is provided a composition for modifying a T cell, the composition comprising: a protein complex comprising a polynucleotide-modifying enzyme domain, a T cell membrane binding domain and an endosome escape domain; a guide oligonucleotide specific to a T cell receptor constant (TRAC) gene of the T cell; and a donor DNA comprising two homology arms at each end of the donor DNA homologous to exon1 of the TRAC gene and encoding therebetween a chimeric antigen T cell receptor comprising: translocation signal for translocation to a cell membrane of the T cell; a transmembrane domain; an intracellular signaling domain; and an extracellular antigen binding domain.

Abstract: There is provided a polynucleotide-modifying enzyme with a functional nuclease domain and a display domain. The functional nuclease domain comprises a nuclease catalytic pocket. The display domain comprises a peptidic recognition sequence of from 3 to 20 amino acids in length, in a loop, an alpha helix or an extension off the end of the alpha helix that is positioned on an external surface of the polynucleotide-modifying enzyme. The peptidic recognition sequence recognizes a target cell receptor of a target cell to allow cell internalization of the polynucleotide-modifying enzyme in said target cell.

Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.

Abstract: Described herein are methods, compositions, and systems for gene editing using polynucleotide modifying enzymes that do not require the use of chemical transfection agents for entry into cells.

Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.

Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.

Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.

Abstract: A bionanofluid includes a carbon-based nanomaterial substantially mono-dispersed in a fluid. The carbon-based nanomaterial is surface modified with a polar group when the fluid is polar or with a non-polar group when the fluid is non-polar, and functionalized with a biological targeting moiety to allow specific association of the carbon-based nanomaterial to a targeted entity. A hybrid bionanofluid includes the bionanofluid, with the carbon-based nanomaterial further modified with a hybrid nanoparticle which includes an alloy, transition metal, semi-conductor, semi-metal or polymer based nanoparticle with biological targeting moiety. A hydrogel, foam, cream, spray or dried product includes the bionanofluid or hybrid bionanofluid. The bionanofluid or hybrid bionanofluid are useful in multimodal imaging (photo-luminescence, luminescence, photo-acoustic, MRI, ultrasound) and/or cellular targeting.

Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.

Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.

Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.

Abstract: A bionanofluid includes a carbon-based nanomaterial substantially mono-dispersed in a fluid. The carbon-based nanomaterial is surface modified with a polar group when the fluid is polar or with a non-polar group when the fluid is non-polar, and functionalized with a biological targeting moiety to allow specific association of the carbon-based nanomaterial to a targeted entity. A hybrid bionanofluid includes the bionanofluid, with the carbon-based nanomaterial further modified with a hybrid nanoparticle which includes an alloy, transition metal, semi-conductor, semi-metal or polymer based nanoparticle with biological targeting moiety. A hydrogel, foam, cream, spray or dried product includes the bionanofluid or hybrid bionanofluid. The bionanofluid or hybrid bionanofluid are useful in multimodal imaging (photo-luminescence, luminescence, photo-acoustic, MRI, ultrasound) and/or cellular targeting.

Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.