Abstract: Pretreatment of biomass using targeted alkaline hydrolysis with optional alkali recovery is shown, where the biomaterial is steam exploded before an alkaline hydrolysis by a) Providing a wetted biomass, b) Flashing the wetted biomass into a pretreatment reactor at a selected pressure, c) Subjecting the biomass to thermal hydrolysis and steam explosion after holding the biomass at a temperature for 10-45 minutes, d) Subjecting the steam exploded biomass to an alkaline hydrolysis by adding an alkali component in a selected concentration obtaining an alkali reacted biomass slurry. Apparatus is also shown having a conditioning unit (B), a feed sluice (C) configured to receive conditioned biomass and guide the biomass to the inlet of a pretreatment reactor/tank (D) for thermal hydrolysis/steam explosion, a flash tank (E), a hydrolysis tank (F) for alkaline hydrolysis, and a separation unit (G) in which the pretreated slurry can be separated into liquid and solid.

Abstract: A novel microorganism is provided named Aspergillus saccharolyticus. Further, beta-glucosidase enzymes encoded by said microorganism are provided, and the use thereof in the degradation of lignocellulosic material. Also, host organisms comprising the polypeptides of the invention and/or polynucleotides encoding these are provided. In addition, methods, compositions, and kit-of-parts are provided which comprise any component of the invention, and optionally any additional components.

Abstract: A novel microorganism is provided named Aspergillus saccharolyticus. Further, beta-glucosidase enzymes encoded by said microorganism are provided, and the use thereof in the degradation of lignocellulosic material. Also, host organisms comprising the polypeptides of the invention and/or polynucleotides encoding these are provided. In addition, methods, compositions, and kit-of-parts are provided which comprise any component of the invention, and optionally any additional components.

Abstract: A novel microorganism is provided named Aspergillus saccharolyticus. Further, beta-glucosidase enzymes encoded by said microorganism are provided, and the use thereof in the degradation of lignocellulosic material. Also, host organisms comprising the polypeptides of the invention and/or polynucleotides encoding these are provided. In addition, methods, compositions, and kit-of-parts are provided which comprise any component of the invention, and optionally any additional components.

Abstract: A novel microorganism is provided named Aspergillus saccharolyticus. Further, beta-glucosidase enzymes encoded by said microorganism are provided, and the use thereof in the degradation of lignocellulosic material. Also, host organisms comprising the polypeptides of the invention and/or polynucleotides encoding these are provided. In addition, methods, compositions, and kit-of-parts are provided which comprise any component of the invention, and optionally any additional components.