Abstract: Positioning structures in at least one indentation present on a or in a support, wherein said indentation is an indentation having a diameter in the nanometer range, makes it possible to position the structure in the indentation substantially centrally with defined orientation. A support having at least one indentation, wherein the indentation has a size in the nanometer range, includes a predetermined structure which is arranged substantially centrally within said indentation and which optionally has a functional unit diametrically opposite to the side pointing to the bottom surface. The arrangement is especially suitable for single molecule analysis and, here especially, for single molecule sequencing and other high-throughput methods.

Abstract: Positioning structures in at least one indentation present on a or in a support, wherein said indentation is an indentation having a diameter in the nanometer range, makes it possible to position the structure in the indentation substantially centrally with defined orientation. A support having at least one indentation, wherein this is one having a size in the nanometer range, includes a predetermined structure which is arranged substantially centrally within said indentation and which optionally has a functional unit diametrically opposite to the side pointing to the bottom surface. The arrangement is especially suitable for single molecule analysis and, here especially, for single molecule sequencing and other high-throughput methods.

Abstract: Arrays which utilize labeling molecules for calibrating a measuring device, such as microscopes, have a first structure based on a DNA origami as a calibration sample, wherein the DNA origami is formed into a predetermined structure by short DNA segments. The DNA origami is optionally present in an arranged manner on a support, wherein a number of short DNA segments which form the predetermined structure include a labeling molecule. Optionally, the array can have at least a second structure based on a DNA origami, different from the first structure, as a calibration sample. The array allows quantification of the labeling molecules on the basis of the number of photons per unit time.

Abstract: The present invention relates generally to signal enhancement in single-molecule-assays. In particular, the present invention relates to an array allowing signal enhancement of single molecules. The assay is in particular a Bioassay allowing single molecule analysis of biomolecules. Preferably, the signal enhancement is a fluorescence enhancement based on optical antenna. In another aspect, the present invention relates to a method for measuring irradiation of electromagnetic waves of single compounds. Based on the arrays and methods, it is possible to allow signal enhancement and, consequently, allow single molecule assays with high sensitivity and reproducibility.

Abstract: The present invention relates to a process for improving the photostability and/or control of the fluorescence intensity of a fluorescent dye wherein a fluorescent dye is admixed with a redox buffer comprising at least one reducing agent and/or at least one oxidizing agent and/or at least one reducing-oxidizing agent, and also to a fluorescent dye composition comprising a fluorescent dye and a redox buffer comprising at least one reducing agent and at least one oxidizing agent or at least one reducing-oxidizing agent.