Abstract: There is provided a device (1) for carrying out an assay to detect a target nucleic acid in a sample, the device (1) comprising a body in which is formed: •a) a sample entry well location comprising a sample entry well (22) or means for engaging with a sample entry well (22), the sample entry well (22) having a first volume; •b) an amplification well location comprising an amplification well (56) or means for engaging with an amplification well (56), the well (56) having a second volume less than or the same as the first volume and in which a nucleic acid amplification reaction of the target nucleic acid may be effected; •c) a first channel (60) linking the sample entry well (22) with the amplification well (56); •d) a diluent well location comprising a diluent well (35) or means for engaging with a diluent well (35), the well (35) being sealed, having a third volume greater than the second volume and being openable to an unsealed configuration; •e) a second channel (65) linking the diluent well (35), when in

Abstract: There is provided a device (1) for carrying out an assay to detect a target nucleic acid in a sample, the device (1) comprising a body in which is formed: • a) a sample entry well location comprising a sample entry well (22) or means for engaging with a sample entry well (22), the sample entry well (22) having a first volume; • b) an amplification well location comprising an amplification well (56) or means for engaging with an amplification well (56), the well (56) having a second volume less than or the same as the first volume and in which a nucleic acid amplification reaction of the target nucleic acid may be effected; • c) a first channel (60) linking the sample entry well (22) with the amplification well (56); • d) a diluent well location comprising a diluent well (35) or means for engaging with a diluent well (35), the well (35) being sealed, having a third volume greater than the second volume and being openable to an unsealed configuration; • e) a second channel (65) linking the diluent well (35), wh

Abstract: A device (1) for carrying out a chemical or biochemical reaction and detecting the results is described. The device may be used in an assay to detect a target nucleic acid in a sample. The device includes (i) a well (8) in which a chemical or biochemical reaction such as a nucleic acid amplification reaction may be effected in a liquid phase or a receiver for such a well; (ii) a channel (16) extending from the well; (iii) a lateral flow assay device (32) arranged to receive liquid contents from channel, on a bibulous membrane thereon. The membrane contains elements that are able to detect the products of the chemical or biochemical reaction such as a target nucleic acid. Methods for using such devices and apparatus for carrying out assays using these are also described and claimed.

Abstract: A device (1) for carrying out a chemical or biochemical reaction and detecting the results, such as an assay to detect a target nucleic acid in a sample, said device comprising (i) a first well (8) in which a chemical or biochemical reaction such as a nucleic acid amplification reaction may be effected in a liquid phase or a receiving means for such a first well; (ii) a first channel (16) extending from the first well; (iii) a lateral flow assay device (32) arranged to receive liquid contents from said first channel, optionally by way of second well, on a bibulous membrane thereon, wherein said membrane contains elements that are able to detect the products of the chemical or biochemical reaction such as a target nucleic acid. Methods for using such devices and apparatus for carrying out assays using these are also described and claimed.