Patents by Inventor Philip Weyman
Philip Weyman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230159955Abstract: The present disclosure relates to compositions and methods of generating circular-permuted nucleic acids for direct use in homology directed genome editing. The method also allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be directly used in a variety of applications without further cloning steps that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.Type: ApplicationFiled: April 16, 2021Publication date: May 25, 2023Inventors: Kunal MEHTA, Philip WEYMAN, Solomon STONEBLOOM
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Patent number: 11279940Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.Type: GrantFiled: May 27, 2021Date of Patent: March 22, 2022Assignee: Zymergen Inc.Inventors: Stephen Blaskowski, Sara da Luz Areosa Cleto, Cameron Coates, Aaron Miller, Sharon Nademanee, Melissa Netwal, Kedar Patel, Shawn Szyjka, Philip Weyman, Solomon Henry Stonebloom, Colin Scott Maxwell, Elizabeth Lauren Meier
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Publication number: 20210324378Abstract: The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.Type: ApplicationFiled: June 23, 2021Publication date: October 21, 2021Inventors: Erik Jedediah DEAN, Kedar PATEL, Aaron MILLER, Kunal MEHTA, Philip WEYMAN
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Publication number: 20210292774Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.Type: ApplicationFiled: May 27, 2021Publication date: September 23, 2021Inventors: Stephen BLASKOWSKI, Sara da Luz Areosa CLETO, Cameron COATES, Aaron MILLER, Sharon NADEMANEE, Melissa NETWAL, Kedar PATEL, Shawn SZYJKA, Philip WEYMAN, Solomon Henry STONEBLOOM, Colin Scott MAXWELL, Elizabeth Lauren MEIER
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Publication number: 20210285014Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.Type: ApplicationFiled: May 27, 2021Publication date: September 16, 2021Inventors: Stephen Blaskowski, Sara da Luz Areosa Cleto, Cameron Coates, Aaron Miller, Sharon Nademanee, Melissa Netwal, Kedar Patel, Shawn Szyjka, Philip Weyman, Solomon Henry Stonebloom, Colin Scott Maxwell, Elizabeth Lauren Meier
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Patent number: 11098279Abstract: Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.Type: GrantFiled: July 2, 2019Date of Patent: August 24, 2021Assignee: ZYMERGEN INC.Inventors: Sean Poust, Vytas SunSpiral, William Serber, Matthew Jonathan Myers, Sara da Luz Areosa Cleto, Philip Weyman
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Patent number: 11066663Abstract: The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.Type: GrantFiled: October 31, 2019Date of Patent: July 20, 2021Assignee: Zymergen Inc.Inventors: Erik Jedediah Dean, Kedar Patel, Aaron Miller, Kunal Mehta, Philip Weyman
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Patent number: 11053506Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.Type: GrantFiled: March 20, 2020Date of Patent: July 6, 2021Assignee: Zymergen Inc.Inventors: Stephen Blaskowski, Sara da Luz Areosa Cleto, Cameron Coates, Aaron Miller, Sharon Nademanee, Melissa Netwal, Kedar Patel, Shawn Szyjka, Philip Weyman, Solomon Henry Stonebloom, Colin Scott Maxwell, Elizabeth Lauren Meier
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Patent number: 11053515Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.Type: GrantFiled: March 20, 2020Date of Patent: July 6, 2021Assignee: Zymergen Inc.Inventors: Stephen Blaskowski, Sara da Luz Areosa Cleto, Cameron Coates, Aaron Miller, Sharon Nademanee, Melissa Netwal, Kedar Patel, Shawn Szyjka, Philip Weyman, Solomon Henry Stonebloom, Colin Scott Maxwell, Elizabeth Lauren Meier
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Publication number: 20200283780Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.Type: ApplicationFiled: March 20, 2020Publication date: September 10, 2020Inventors: Stephen BLASKOWSKI, Sara da Luz Areosa CLETO, Cameron Coates, Aaron Miller, Sharon Nademanee, Melissa Netwal, Kedar Patel, Shawn Szyjka, Philip Weyman, Solomon Henry Stonebloom, Colin Scott Maxwell, Elizabeth Lauren Meier
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Publication number: 20200283802Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.Type: ApplicationFiled: March 20, 2020Publication date: September 10, 2020Inventors: Stephen BLASKOWSKI, Sara da Luz Areosa Cleto, Cameron Coates, Aaron Miller, Sharon Nademanee, Melissa Netwal, Kedar Patel, Shawn Szyjka, Philip Weyman, Solomon Henry Stonebloom, Colin Scott Maxwell, Elizabeth Lauren Meier
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Publication number: 20200131508Abstract: The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.Type: ApplicationFiled: October 31, 2019Publication date: April 30, 2020Inventors: Erik Jedediah Dean, Kedar Patel, Aaron Miller, Kunal Mehta, Philip Weyman
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Publication number: 20200032356Abstract: Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.Type: ApplicationFiled: July 2, 2019Publication date: January 30, 2020Inventors: Sean Poust, Vytas SunSpiral, William Serber, Matthew Jonathan Myers, Sara da Luz Areosa Cleto, Philip Weyman
