Abstract: The present invention is related to a reversible, parallel and/or multitask cloning method and kit, which improve the cloning of (preferably multiple) genetic element(s) in a nucleic acid construct such as vector or in chromosome of a cell and the rapid and efficient selection of said construct with a correct integration of said genetic element(s) either in vitro or in vivo.

Abstract: The present invention is related to a method for a high through-put screening detection of genetic modifications in genome engineering and a system for homologous recombination of an exogenous nucleotide sequence into a target cell genome sequence.

Abstract: A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.

Abstract: A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.

Abstract: The present invention is related to specific sequences, preferably present in a diagnostic kit to identify if a female mammal comprises in her genome a mutation in the alpha-fetoprotein sequence or a partial or total deletion of this alpha-fetoprotein sequence, present heterozygously or homozygously (on both allele).

Abstract: The present invention is related to a method for a high through-put screening detection of genetic modifications in genome engineering and a system for homologous recombination of an exogenous nucleotide sequence into a target cell genome sequence.

Abstract: The present invention is related to a reversible, parallel and/or multitask cloning method and kit, which improve the cloning of (preferably multiple) genetic element(s) in a nucleic acid construct such as vector or in chromosome of a cell and the rapid and efficient selection of said construct with a correct integration of said genetic element(s) either in vitro or in vivo.

Abstract: The present invention is related to a non-human genetically modified mammal comprising a mutation, a partial or total deletion of the genetic sequence encoding the wild type mammal alpha-fetoprotein.

Abstract: The present invention is related to a recombinant eucaryote cell or organism having incorporated in its genome a genetic construct made of at least one nucleotide sequence encoding a toxic gene (TOX) under the control of an inducible promoter/operator genetic sequence and possibly a selectable marker. The present invention is also related to a production and selection method-of-genetically modified eucaryote cells or organisms having integrated into their genome foreigner (exogenous) DNA fragment(s) by using said recombinant eucaryote cells or organisms.

Abstract: A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.

Abstract: The present invention is related to a method for the selection of recombinant clones having integrated a gene of interest (6) and a nucleotide sequence (3) encoding a functional antidote protein (4) to a toxic molecule (5), wherein said recombinant clones are the ones which survive following their integration into a host cell (20) comprising in its genome a nucleotide sequence (21) encoding said toxic molecule (5). The present invention is also related to a nucleic acid construct, a vector comprising said nucleic acid construct, a host cell and a cloning and/or sequencing kit for performing said method.

Abstract: The present invention is related to a nucleic acid construct (1) to be incorporated in a double selection vector (2) able to transform a cell (3) of a specific organism, wherein—said construct (1) contains two different genes (10 and 11), each gene encoding a different toxic molecule (4 and 5) to a prokaryote cell, preferably to E. Coli, said genes (10 and 11) being disposed upstream and downstream a cassette sequence (8), or downstream and upstream site(s) for the insertion of a cassette sequence (8), and—said nucleic acid construct comprises specific sequence portions (12, 12′) allowing inactivation of said genes (10 and 11).

Abstract: A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.

Abstract: A cloning and/or sequencing vector (1) comprises a nucleotide promoter sequence (3) and a nucleotide sequence (4) coding for a fusion protein active as a poison, which nucleotide sequence (4) is obtained by fusion of a coding nucleotide sequence (5) containing several unique cloning sites with a nucleotide sequence (6) coding for a poison protein, wherein the nucleotide sequences (3) and (4) are incorporated in an autonomous replication vector (2).