Abstract: A method of lyophilizing a target material includes placing a container with a target material in a lyophilizer of a lyophilization system. The lyophilization system includes the lyophilizer, a refrigeration system coupled to the lyophilizer, a temperature controller coupled to the refrigeration system, and a flow path connecting the lyophilizer and the refrigeration system. The method includes activating the lyophilizer where the lyophilizer includes a plurality of walls defining an interior chamber and at least one shelf disposed in the interior chamber. The method includes running a freezing cycle, running a primary drying cycle, and running a secondary drying cycle. The method includes pumping a thermal fluid through the flow path in fluid communication with the at least one shelf and at least one wall. Running the freezing cycle includes pumping the thermal fluid through the at least one wall of the plurality of walls.

Abstract: The invention is directed to formation and use of electroprocessed fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed fibrin may also be combined with other molecules in order to deliver the molecules to the site of application or implantation of the electroprocessed fibrin. The fibrin or fibrin/cell suspension is electrodeposited onto a substrite to form the tissues and organs.

Abstract: The invention is directed to formation and use of electroprocessed fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed fibrin may also be combined with other molecules in order to deliver the molecules to the site of application or implantation of the electroprocessed fibrin. The fibrin or fibrin/cell suspension is electrodeposited onto a substrate to form the tissues and organs.

Abstract: A method of cooling a biological material includes providing a vessel. The vessel includes a generally vertical inner wall disposed at an inner radius from a vertically disposed center axis. The generally vertical inner wall defines in part an interior volume. A medium including a biological material is provided. A plurality of heat transfer surfaces is positioned within the interior volume. Each of the plurality of heat transfer surfaces is oriented generally vertically through the interior volume. Each of the heat transfer surfaces is arranged such that it does not extend into the portion of the interior volume defined by a distance from the center axis greater than 90% of the inner radius. The medium is introduced into the vessel. A heat transfer fluid is circulated through the plurality of heat transfer surfaces. The heat transfer surfaces conduct heat out of the medium.

Abstract: The invention is directed to formation and use of electroprocessed fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed fibrin may also be combined with other molecules in order to deliver the molecules to the site of application or implantation of the electroprocessed fibrin. The fibrin or fibrin/cell suspension is electrodeposited onto a substrate to form the tissues and organs.

Abstract: The invention is directed to use of fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or “organ-like” tissue. A preferred embodiment is a plasma-derived fibrin matrix containing cells.

Abstract: The invention is directed to use of fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or “organ-like” tissue. A preferred embodiment is a plasma-derived fibrin matrix containing cells.

Abstract: The invention is directed to formation and use of electroprocessed fibrin as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed fibrin may also be combined with other molecules in order to deliver the molecules to the site of application or implantation of the electroprocessed fibrin. The fibrin or fibrin/cell suspension is electrodeposited onto a substrate to form the tissues and organs.

Abstract: A method of separating a material from a liquid sample comprising:providing a system for material separation having a stationary phase having a metalloporphyrin coordination compound or a metallophthalocyanine coordination compound or a mixture thereof;oxidizing or reducing the coordination compound, respectively, to an oxidized or reduced state at which the material will bind to the compound;applying a source of electric potential to the system; andcontacting the oxidized or reduced coordination compound with a liquid sample containing the material under conditions effective to separate the material from the liquid.