Abstract: A method is for discontinuous plasmid extraction from a microorganism and for purifying a plasmid of interest. The method includes adding a neutralizing solution and then a precipitation solution via a plurality of orifices, under mild mechanical stirring.

Abstract: A method is for purifying a biomolecule (protein, DNA) of interest from a culture of Gram-negative bacteria uses polyoxyethylene isooctylcyclohexyl ether to remove endotoxins. Polyoxyethylene isooctylcyclohexyl ether is used to remove endotoxins and a solution having a pH between 8.0 and 9.0 and a concentration of salts from 2.5M to 3M in chromatography is used for specific leaching of bacterial contaminants.

Abstract: A method is for purifying a biomolecule (protein, DNA) of interest from a culture of Gram-negative bacteria uses polyoxyethylene isooctylcyclohexyl ether to remove endotoxins. Polyoxyethylene isooctylcyclohexyl ether is used to remove endotoxins and a solution having a pH between 8.0 and 9.0 and a concentration of salts from 2.5M to 3M in chromatography is used for specific leaching of bacterial contaminants.

Abstract: A satellite electric propulsion power supply unit comprises: an internal source of electrical power; an external input adapted to receive an electrical power supply from an external source of electrical power; a first external output and a second external output adapted to deliver as output, respectively, a first electrical power supply and a second electrical power supply; a first selector element and a second selector element such that: the first selector element is equipped with a first internal input connected to the internal source and with two outputs: the first external output and an internal output connected to a second internal input of the second selector element; and the second selector element is equipped with an output corresponding to the second external output and with two inputs: the external input and the second internal input.

Abstract: A satellite electric propulsion power supply unit comprises: an internal source of electrical power; an external input adapted to receive an electrical power supply from an external source of electrical power; a first external output and a second external output adapted to deliver as output, respectively, a first electrical power supply and a second electrical power supply; a first selector element and a second selector element such that: the first selector element is equipped with a first internal input connected to the internal source and with two outputs: the first external output and an internal output connected to a second internal input of the second selector element; and the second selector element is equipped with an output corresponding to the second external output and with two inputs: the external input and the second internal input.

Abstract: An apparatus and a method for obtaining a (poly)nucleotide sequence of interest include steps of cultivating hosts cells to produce a nucleotide sequence of interest and harvesting these cells, introducing these cells in a passageway and disintegrating them in a continuous process. In the continuous process, performing in the passageway a precipitation of contaminants by a mixing of the disintegrated cells with a solution containing one or more salt(s) and obtaining a mixture and allowing a precipitate to separate from the solution of this mixture, preferably to float and/or to sediment from the solution of this mixture for 1-48 hours and pumping out a soluble material from this solution, while excluding recovering the precipitate.

Abstract: An apparatus and a method for obtaining a (poly)nucleotide sequence of interest include steps of cultivating hosts cells to produce a nucleotide sequence of interest and harvesting these cells, introducing these cells in a passageway and disintegrating them in a continuous process. In the continuous process, performing in the passageway a precipitation of contaminants by a mixing of the disintegrated cells with a solution containing one or more salt(s) and obtaining a mixture and allowing a precipitate to separate from the solution of this mixture, preferably to float and/or to sediment from the solution of this mixture for 1-48 hours and pumping out a soluble material from this solution, while excluding recovering the precipitate.

Abstract: An apparatus and a method for obtaining a (poly)nucleotide sequence of interest include steps of cultivating hosts cells to produce a nucleotide sequence of interest and harvesting these cells, introducing these cells in a passageway and disintegrating them in a continuous process. In the continuous process, performing in the passageway a precipitation of contaminants by a mixing of the disintegrated cells with a solution containing one or more salt(s) and obtaining a mixture and allowing a precipitate to separate from the solution of this mixture, preferably to float and/or to sediment from the solution of this mixture for 1-48 hours and pumping out a soluble material from this solution, while excluding recovering the precipitate.

Abstract: An apparatus and a method for obtaining a (poly) nucleotide sequence of interest include steps of cultivating hosts cells to produce a nucleotide sequence of interest and harvesting these cells, introducing these cells in a passageway and disintegrating them in a continuous process. In the continuous process, performing in the passageway a precipitation of contaminants by a mixing of the disintegrated cells with a solution containing one or more salt(s) and obtaining a mixture and allowing a precipitate to separate from the solution of this mixture, preferably to float and/or to sediment from the solution of this mixture for 1-48 hours and pumping out a soluble material from this solution, while excluding recovering the precipitate.