Abstract: Disclosed is a process of detecting at least one molecule of interest in a sample, including the steps of: i) putting the sample in contact with a ligand that is specific to this molecule of interest and that is coupled with a fluorochrome consisting of phycoerythrin or one of its derivatives, ii) excitation of the mixture from step i) by at least one light source with a wavelength between 330 and 425 nm, iii) detecting an emission of light by the mixture following step ii) at a wavelength greater than or equal to 550 nm, and iv) determining whether the molecule of interest is present in the sample in view of the results of step iii).

Abstract: The invention relates to a method for the detection and multiplex quantification of analytes in a sample, using functionalised microspheres, whereby said microspheres are magnetised after the sample has been brought into contact therewith. The inventive method is particularly suitable for the detection and multiplex quantification of several analytes by means of flow cytometry. The invention also relates to a kit which is used for the detection and/or quantification of several analytes in order to carry out the inventive method, comprising a suspension of functionalised non-magnetic microspheres, a ferrofluid solution and a solution with at least one conjugate.