Abstract: The invention concerns a method for the detection of Ralstonia solanacearum race 3 biovar 2 strains in a medium, comprising the determination of the presence or the absence in a sample of the medium, of: (i) at least one first nucleic acid target having a sequence selected from the group constituted of SEQ ID NO: 1-49, complementary sequences thereof, and homologous sequences thereof, or (ii) at least one fragment of said first target nucleic acid, wherein said fragment is not constituted of or comprised in a sequence selected from the group constituted of SEQ ID NO: 111-140; whereby, if said first nucleic acid target or fragment thereof is present in the sample, it is determined that Ralstonia solanacearum race 3 biovar 2 strain is present in the medium.