Abstract: A gel casting system having a filling manifold and a gel casting clamp. The gel casting clamp is a U-shaped boot made of a flexible and resilient material and having a tapered channel disposed on its inner surface. The tapered channel secures a gel mold in the gel casting clamp. The gel casting clamp is disposed on top of the filling manifold, and an inlet in the bottom of the gel casting clamp mates with an outlet on the filling manifold to allow fluid to enter the gel mold from the filling manifold. The filling manifold includes two plates detachably connected to each other with a distribution system disposed between the plates. The filling manifold also includes an intake port through which fluid is supplied to the filling manifold from a supply means. In one embodiment, the supply means is a gradient former.